Zhan-ying Ma, B. Yao, Liansheng Wang, Yang-qing He
{"title":"山茱萸化学成分及其生物活性研究","authors":"Zhan-ying Ma, B. Yao, Liansheng Wang, Yang-qing He","doi":"10.4103/2229-5186.99579","DOIUrl":null,"url":null,"abstract":"Objective: To study the chemical constituents from Cornus officinalis Sieb., Et Zucc, and their peroxisome proliferator-activated receptors (PPARs) agonist activity. Materials and Methods: The leaves of C. officinalis were extracted three times with 90% EtOH at room temperature. The ethanol extracts were combined and concentrated under reduced pressure to yield residue, which was isolated and purified by silica gel and reverse-phase C 18 column chromatography. The structures were elucidated on the basis of spectroscopic evidence and their physiochemical characteristics. Cell-based luciferase reporter gene assays were used to evaluate PPARα/γ agonistic activities. Results: Five compounds were isolated and elucidated as 10-hydroxyhastatoside (1), β-dihydrocornin (2), isoquercitrin (3), loganin (4) and oleanolic acid (5). Conclusion: Compounds 1 and 2 were obtained from C. officinalis for the first time. Compound 3 exhibited moderate agonistic activities for PPARα, with EC 50 values of 29.5 μM.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":"89 1","pages":"209"},"PeriodicalIF":0.0000,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Chemical constituents from Cornus officinalis and their biological activity 1\",\"authors\":\"Zhan-ying Ma, B. Yao, Liansheng Wang, Yang-qing He\",\"doi\":\"10.4103/2229-5186.99579\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: To study the chemical constituents from Cornus officinalis Sieb., Et Zucc, and their peroxisome proliferator-activated receptors (PPARs) agonist activity. Materials and Methods: The leaves of C. officinalis were extracted three times with 90% EtOH at room temperature. The ethanol extracts were combined and concentrated under reduced pressure to yield residue, which was isolated and purified by silica gel and reverse-phase C 18 column chromatography. The structures were elucidated on the basis of spectroscopic evidence and their physiochemical characteristics. Cell-based luciferase reporter gene assays were used to evaluate PPARα/γ agonistic activities. Results: Five compounds were isolated and elucidated as 10-hydroxyhastatoside (1), β-dihydrocornin (2), isoquercitrin (3), loganin (4) and oleanolic acid (5). Conclusion: Compounds 1 and 2 were obtained from C. officinalis for the first time. Compound 3 exhibited moderate agonistic activities for PPARα, with EC 50 values of 29.5 μM.\",\"PeriodicalId\":10187,\"journal\":{\"name\":\"Chronicles of Young Scientists\",\"volume\":\"89 1\",\"pages\":\"209\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chronicles of Young Scientists\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/2229-5186.99579\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chronicles of Young Scientists","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/2229-5186.99579","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Chemical constituents from Cornus officinalis and their biological activity 1
Objective: To study the chemical constituents from Cornus officinalis Sieb., Et Zucc, and their peroxisome proliferator-activated receptors (PPARs) agonist activity. Materials and Methods: The leaves of C. officinalis were extracted three times with 90% EtOH at room temperature. The ethanol extracts were combined and concentrated under reduced pressure to yield residue, which was isolated and purified by silica gel and reverse-phase C 18 column chromatography. The structures were elucidated on the basis of spectroscopic evidence and their physiochemical characteristics. Cell-based luciferase reporter gene assays were used to evaluate PPARα/γ agonistic activities. Results: Five compounds were isolated and elucidated as 10-hydroxyhastatoside (1), β-dihydrocornin (2), isoquercitrin (3), loganin (4) and oleanolic acid (5). Conclusion: Compounds 1 and 2 were obtained from C. officinalis for the first time. Compound 3 exhibited moderate agonistic activities for PPARα, with EC 50 values of 29.5 μM.