An Overview on the Main Technical Processes of Restriction-Site Associated DNA Sequencing (RAD-Seq)

Restriction site-associated DNA sequencing (RAD-seq) is a critical enabling genotyping method based on whole-genome restriction sites developed on the basis of second-generation sequencing and nowadays is widely applicable to a variety of biological problems in many species where traditional cytogenetic methods have failed. As sequencing costs have dropped, RAD-Seq has moved from being primarily a SNP discovery tool to being a method for high-throughput genotyping of populations. In this paper, the main technical processes of RAD-seq has been briefly summarized and great potential will been hold to clarify more development and application in researches.