斑马鱼(Danio rerio)生殖腺急性暴露于新烟碱类杀虫剂吡虫啉:组织病理学方法

IF 0.9 4区 环境科学与生态学 Q4 LIMNOLOGY Annales De Limnologie-international Journal of Limnology Pub Date : 2021-01-01 DOI:10.1051/limn/2021021
C. Akbulut
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引用次数: 1

摘要

新烟碱类杀虫剂是一类对昆虫具有高度靶向特异性的新型杀虫剂。吡虫啉是一种新烟碱类杀虫剂,是世界上使用最广泛的杀虫剂。由于吡虫啉在农业中的广泛使用,对水生系统造成干扰,对水生环境造成威胁。本研究旨在探讨吡虫啉对斑马鱼性腺的组织病理学影响。考虑96 h LC50值,斑马鱼分别暴露于9.5 mg/L、19 mg/L和38 mg/L吡虫啉5天。解剖性腺组织后,应用常规组织学技术,对组织进行周期性酸-希夫(PAS)、甲苯胺蓝(TB)、血红素和伊红(H&E)染色。切片在光镜下检查。在对照组性腺组织学正常的情况下,实验组睾丸组织出现了组织病理学改变,如精小管变性和结合、生精细胞和间质细胞肥大、间质纤维化等。实验组卵巢组织可见卵母细胞结构退化、自溶、闭锁卵母细胞数量增加、皮质泡空泡化、辐射带增厚卷曲、卵泡周围层开口。
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Acute Exposure to the Neonicotinoid Insecticide Imidacloprid of Zebrafish (Danio rerio) Gonads: A Histopathological Approach
Neonicotinoids are the new class of insecticides that are high target specificity to insects. Imidacloprid is a neonicotinoid that is the most widely used insecticide in the world. As a result of its widespread use in agriculture, imidacloprid interferes with the aquatic system and threatens the aquatic environment. In this study, an investigation of the histopathological effects of imidacloprid on zebrafish gonads was aimed. Zebrafish were exposed to 9.5 mg/L, 19 mg/L, and 38 mg/L of imidacloprid for 5 days, considering the 96 h LC50 value. After dissecting the gonadal tissues, routine histological techniques were applied, and the tissues were stained with Periodic Acid-Schiff (PAS), Toluidine Blue (TB), and Haematoxylin and Eosin (H&E). Sections were examined under a light microscope. While normal gonad histology was observed in the control group, histopathological alternations such as degeneration and union in the seminiferous tubules, hypertrophy in spermatogenic and Leydig cells, and interstitial fibrosis were detected in testicular tissue of the experimental groups. In the ovarian tissues of the experimental groups, structural deterioration in oocytes, autolysis, increase in the number of atretic oocytes, vacuolization in cortical alveoli, thickening and curling in the zona radiata, and opening in the perifollicular layers were detected.
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来源期刊
CiteScore
2.20
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Annales de Limnologie - International Journal of Limnology publishes papers on the ecology of freshwater systems, ranging from studies of aquatic organisms, physical and chemical works which relate to the biological environment, to ecological applications and frameworks for water management directives. Main topics: Ecology of freshwater systems ; biodiversity, taxonomy, distribution patterns in space and time, biology of animals and plants ; experimental and conceptual studies which integrate laboratory and/or field work on physiology, population dynamics, biogeochemistry and nutrient dynamics, management, mathematical modelling ; techniques for sampling and chemical analyses, ecological applications, procedures which provide frameworks for environmental legislation.
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