黄芩苷诱导MCF-7乳腺癌细胞辐射耐药

M. Rouhani
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引用次数: 0

摘要

目标:乳腺癌在全世界妇女中发病率和与癌症相关的死亡率最高。放疗用于治疗不同阶段的乳腺癌。几种多酚增加了放射治疗的有效性。黄芩苷是黄芩中富含的一种黄酮类化合物。该化合物能明显诱导癌细胞凋亡和抑制癌细胞增殖。我们研究了沃戈宁对典型乳腺癌细胞系电离辐射反应的影响。方法:采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑法测定MCF-7细胞活力。细胞计数和双染色细胞凋亡实验也被用来寻找合适的生理相关浓度的沃戈宁。用沃戈宁处理的细胞暴露在不同剂量的x射线下,用克隆生存试验确定沃戈宁对辐射后存活的影响。结果:Wogonin降低MCF-7细胞活力呈剂量和时间依赖性。剂量依赖性地减少细胞数量,增加凋亡细胞百分比。辐射前3天用5µM和10µM浓度的沃戈宁预处理的细胞与未用沃戈宁处理的细胞相比,显示出更高的辐射抗性。结论:wogonin对MCF-7乳腺癌细胞具有较强的抗电离辐射能力。
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Wogonin from Scutellaria baicalensis-Induced Radioresistance in MCF-7 Breast Cancer Cell Line
nu-Objectives: Breast cancer shows the highest incidence and cancer-related deaths among women worldwide. Radiotherapy is used for treating different stages of breast cancer. Several polyphenols have increased the effectiveness of radiotherapy. Wogonin is a flavone compound abundant in the root of Chinese skullcap. This compound induced apoptosis and inhibited proliferation distinctively in cancer cells. We studied effect of wogonin on the response of a typical breast cancer cell line to ionizing radiation. Methods: MCF-7 cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell counting and double staining apoptosis assays were also used to find suitable physiologically relevant concentrations of wogonin. Cells treated with wogonin were exposed to different doses of X-ray and clonogenic survival assay was utilized to determine the effect of wogonin on survival from radiation. Results: Wogonin treatment decreased the viability of MCF-7 cells in a dose- and time-dependent manner. It decreased number of cells and increased percent of apoptotic cells dose dependently. Cells pretreated with 5 and 10 µM concentrations of wogonin 3 days before radiation showed increased radioresistance compared with cells that were not treated with wogonin. Conclusion: Treatment of MCF-7 breast cancer cells with wogonin made them more resistant to ionizing radiation.
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