过滤法从模拟水系统中分离好氧异养和厌氧硫酸盐还原菌

Esra Ilhan Sungur, Bihter Minnoş, Nihal Dogruoz
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引用次数: 7

摘要

本研究的目的是比较过滤法与直接法(不过滤)检测和计数好氧异养菌或厌氧硫酸盐还原菌(SRB)的效果。此外,我们还比较了滤布平板实验和铺布平板实验(即在R2A琼脂平板上镀)对异养菌菌落形态类型的影响。我们检测到未过滤和过滤样品的SRB平均值和异养细菌计数无统计学差异。通过对过滤后样品与未过滤样品的培养和计数成功率进行比较,实验结果清楚地表明,过滤法可以进行SRB和异养菌的培养和计数。此外,从过滤后的样品中回收的不同菌落类型的数量通常高于未过滤的样品。
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Isolation of Aerobic Heterotrophic and Anaerobic Sulphate Reducing Bacteria from Model Water System by Filtration Method
The purpose of this study was to compare the efficacy of the filtration method with direct method (without filtration) for detection and enumeration of either aerobic heterotrophic bacteria or anaerobic sulphate reducing bacteria (SRB). In addition we compared the results of colony morphology types of heterotrophic bacteria performed by filtration-spread plate experiments with spread plate experiments (i.e. plating on R2A agar plates). We detected that there was no statistically significant difference in the mean of SRB and heterotrophic bacteria counts from unfiltered and filtered samples. The results of the experiments comparing the cultivation and enumeration success of the filtered samples with unfiltered samples clearly demonstrate that filtration method enables the cultivation and enumeration of SRB and heterotrophic bacteria. In addition, the numbers of distinct colony types recovered from filtered samples were usually higher than unfiltered samples.
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