Vinolyn Sylvia, Rajendran Joy Salomi, L. Rajendran, M. Lyons
{"title":"循环底物转化安培酶电极非线性过程的理论与数值分析","authors":"Vinolyn Sylvia, Rajendran Joy Salomi, L. Rajendran, M. Lyons","doi":"10.3390/electrochem3010005","DOIUrl":null,"url":null,"abstract":"A theoretical model of amperometric enzyme electrodes has been developed in which chemical amplification occurs in a single enzyme membrane via cyclic substrate conversion. The system is based on non-stationary diffusion equations with a nonlinear factor related to the Michaelis–Menten kinetics of the enzymatic reaction. By solving the nonlinear equations using the AGM technique, simple analytical expressions of concentration substrate, product, and amperometric current response are derived. Further, biosensor sensitivity, resistance, and gain are obtained from the current. MATLAB programming was used to carry out the digital simulation. The analytical results are validated with the numerical results. The effect of substrate concentration, maximum enzymatic rate, and membrane thickness on biosensor response was evaluated.","PeriodicalId":11612,"journal":{"name":"Electrochem","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Theoretical and Numerical Analysis of Nonlinear Processes in Amperometric Enzyme Electrodes with Cyclic Substrate Conversion\",\"authors\":\"Vinolyn Sylvia, Rajendran Joy Salomi, L. Rajendran, M. Lyons\",\"doi\":\"10.3390/electrochem3010005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A theoretical model of amperometric enzyme electrodes has been developed in which chemical amplification occurs in a single enzyme membrane via cyclic substrate conversion. The system is based on non-stationary diffusion equations with a nonlinear factor related to the Michaelis–Menten kinetics of the enzymatic reaction. By solving the nonlinear equations using the AGM technique, simple analytical expressions of concentration substrate, product, and amperometric current response are derived. Further, biosensor sensitivity, resistance, and gain are obtained from the current. MATLAB programming was used to carry out the digital simulation. The analytical results are validated with the numerical results. The effect of substrate concentration, maximum enzymatic rate, and membrane thickness on biosensor response was evaluated.\",\"PeriodicalId\":11612,\"journal\":{\"name\":\"Electrochem\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-01-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Electrochem\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/electrochem3010005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electrochem","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/electrochem3010005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Theoretical and Numerical Analysis of Nonlinear Processes in Amperometric Enzyme Electrodes with Cyclic Substrate Conversion
A theoretical model of amperometric enzyme electrodes has been developed in which chemical amplification occurs in a single enzyme membrane via cyclic substrate conversion. The system is based on non-stationary diffusion equations with a nonlinear factor related to the Michaelis–Menten kinetics of the enzymatic reaction. By solving the nonlinear equations using the AGM technique, simple analytical expressions of concentration substrate, product, and amperometric current response are derived. Further, biosensor sensitivity, resistance, and gain are obtained from the current. MATLAB programming was used to carry out the digital simulation. The analytical results are validated with the numerical results. The effect of substrate concentration, maximum enzymatic rate, and membrane thickness on biosensor response was evaluated.