Validation and development of UV-Vis spectrophotometer analysis methods for alpha-tocopherol acetate

Background: The COVID-19 pandemic had caused the consumption of vitamins in the community to increase with the aim of maintaining immunity in the body. Objective: This study aims to develop an analysis method with a UV-Vis spectrophotometer, which is considered an alternative analysis method that is easier, more practical, and cheaper. Method: Methods for analyzing alpha-tocopherol acetate are validated through specificity tests, linearity measurements, precision tests, accuracy tests, determination of detection limits, determination of quantitation limits, and determination of range using ethanol p.a solvent and UV-Vis Spectrophotometer instruments. Then, examine the alpha tocopherol acetate content of vitamin E dietary supplements. Results: The specificity test showed that matrix had no significant effect on the analysis, the linearity test met the requirements with a concentration range of 20–60 ug/mL (r = 0.9991), the accuracy test was 99.897-100.315%, intraday and interday precision tests were obtained with %RSD less than 2%, the LOD value was 1.859 ug/mL and the LOQ value was 5.635 ug/mL. The determination of levels of vitamin E in dietary supplements showed levels for brand A of 100.508% and brand B of 100.089%. Conclusion: The analytical method developed had met the validation requirements, and the levels of vitamin E food supplements had met the USP requirements. Keywords: method validation, UV-Vis spectrophotometer, alpha tocopherol acetate