Domestication of strain SYSHHJ1 and its biodegradation of cyclohexane

The synergistic domestication and culture conditions are studied and quantity of adding cyclohexanol and cyclohexanone to culture medium is fixed: 0.6mL·L−1 (culture medium). A bacterial strain SYSHHJ1 using cyclohexane as the sole source of carbon and energy was obtained by synergistic domestication culture. And then effect of growth conditions such as culture times, cyclohexane mass concentration, pH, salt concentration and temperature on strain SYSHHJ1 is studied, and orthogonal experiment is designed, the optional culture conditions of the strain were obtained as: culture time is 84∼108 h, cyclohexane mass concentration is 480mg·L−1, pH is 6, salt concentration is 10g·L−1, temperature is 30°C. Metabolic product is analyzed by HPLC and the result proved also that cyclohexane can be utilized by strain SYSHHJ1.