农业废弃物纤维素源水解Hcl和纤维素酶生产乙醇的比较

Indra Masmur, Clairine Clairine, Mega Evelin Rajagukguk, Teti Purwita Sari, E. Sitinjak
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引用次数: 0

摘要

从玉米壳、稻秆和稻壳等农业废弃物中分离纤维素,利用纤维素生产乙醇。采用NaOH 2%预处理脱木质素分离纤维素,分别得到玉米壳16、6921 g(22,25%)、稻草16、9021 g(22,53%)和稻壳16、3691 g(21,85%)纤维素,并用FT-IR和SEM分析其官能团。用30%盐酸和纤维素酶水解最多2g的纤维素得到糖,用Benedict和Tollens试剂定性,用Luff Schoorl法定量,得到的谷壳含糖量最高为8.64%和10.95%。下一阶段水解糖用酵母(Saccharomyces cerevisiae)发酵6天,然后在78-80℃的温度下蒸馏。用重铬酸钾氧化法计算了农业废弃物中乙醇的最高收率,其中乙醇化学法为5.97%,酶法为6.89%。
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Ethanol Production Comparison from Agricultural Waste of Cellulose Source Within Hydrolysis Hcl and Cellulose Enzyme
The production of Ethanol has been utilized through the process of cellulose isolation from agricultural waste such as corn husk, rice straw and rice husk. Isolation of cellulose with pretreatment delignification using, NaOH 2% to produces cellulose of corn husk 16,6921 g (22,25%), rice straw 16,9021 g (22,53%) and rice husk 16,3691 g (21,85%) then analyzed the functional groups using FT-IR and SEM analysis. Cellulose as much as 2 g is hydrolyzed using HCl 30% and cellulase enzymes to obtain sugar that is qualitatively tested with Benedict and Tollens reagent and quantitatively tested with Luff Schoorl method and produces highest sugar content of rice husk 8,64% and 10,95%. The next stage sugar of hydrolysis will be fermented using  yeast (Saccharomyces cerevisiae) with 6 days, and then distilled at a temperature of 78-80oC. Ethanol was calculated by the potassium dichromate oxidation method to gave highest result from agricultral waste of ethanol chemical 5,97% and enzymatic 6,89% is rice husk.
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