Luisa Ferrari, J. Metovic, Daniela Pulcinelli, Antonio Lombardi, Daniel W. Rullo, F. Papola, L. Ventura
{"title":"病理博物馆湿标本中保存液的pH值评价和古代DNA提取","authors":"Luisa Ferrari, J. Metovic, Daniela Pulcinelli, Antonio Lombardi, Daniel W. Rullo, F. Papola, L. Ventura","doi":"10.4081/jbr.2022.10820","DOIUrl":null,"url":null,"abstract":"Pathology museums host ancient samples obtained during autopsies and generally used for educational purposes in the past. Such collections consist of dry and wet specimens showing diseases that no longer exist or with their natural course unmodified by modern therapies.1,2 In wet specimens, the preservation of macroscopic features due to the storage fluid has a great historical and paleopathological interest. Unfortunately, both original fixatives and storage fluids strongly influence tissue antigens and nucleic acids preservation.3 [...]","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"8 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"pH evaluation of storage fluids and ancient DNA extraction from wet specimens in pathology museums\",\"authors\":\"Luisa Ferrari, J. Metovic, Daniela Pulcinelli, Antonio Lombardi, Daniel W. Rullo, F. Papola, L. Ventura\",\"doi\":\"10.4081/jbr.2022.10820\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Pathology museums host ancient samples obtained during autopsies and generally used for educational purposes in the past. Such collections consist of dry and wet specimens showing diseases that no longer exist or with their natural course unmodified by modern therapies.1,2 In wet specimens, the preservation of macroscopic features due to the storage fluid has a great historical and paleopathological interest. Unfortunately, both original fixatives and storage fluids strongly influence tissue antigens and nucleic acids preservation.3 [...]\",\"PeriodicalId\":9116,\"journal\":{\"name\":\"Bollettino della Societa italiana di biologia sperimentale\",\"volume\":\"8 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bollettino della Societa italiana di biologia sperimentale\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4081/jbr.2022.10820\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bollettino della Societa italiana di biologia sperimentale","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4081/jbr.2022.10820","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
pH evaluation of storage fluids and ancient DNA extraction from wet specimens in pathology museums
Pathology museums host ancient samples obtained during autopsies and generally used for educational purposes in the past. Such collections consist of dry and wet specimens showing diseases that no longer exist or with their natural course unmodified by modern therapies.1,2 In wet specimens, the preservation of macroscopic features due to the storage fluid has a great historical and paleopathological interest. Unfortunately, both original fixatives and storage fluids strongly influence tissue antigens and nucleic acids preservation.3 [...]
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