半矮秆Tef [Eragrostis Tef (Zucc.)]的聚类和主成分分析以倒伏为主的重组自交系

Getahun Bekana
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摘要

Tef是埃塞俄比亚广泛生产和消费的主要谷类作物,深受当地数百万小农的喜爱。最近,由于其无麸质谷物和营养价值,它在世界其他地区也获得了粮食作物的认可。倒伏是影响小麦和秸秆产量和品质的主要因素。目前的研究是根据相似性对这些系进行分组,并评估它们之间的遗传距离大小;然后确定个体特征对总变异的贡献。在2017年主要雨季,采用简单格点设计对49个品系的16个性状进行了评价。除单株可育分蘖数外,各品系间均表现出极显著差异,且品系与各品系间的互作效应显著。聚类分析将这些线根据相似性分为四类。簇2和簇4之间的簇间距离最大,而簇1和簇4之间的簇间距离最小。主成分分析表明,5个特征值大于1的主成分可解释约77.6%的总方差。结果表明,4个重组自交系的产量均高于当地和标准检验。RIL 14号表现出最高的籽粒产量和较低的倒伏指数等优良性状,可作为选育材料,为今后的tef育种奠定基础和加强基础;尤其是住宿问题。
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Cluster and Principal Component Analysis of Semi-Dwarf Tef [Eragrostis tef (Zucc.) Trotter] Recombinant Inbred Lines with Emphasis to Lodging
Tef is the main cereal crop widely produced and consumed in Ethiopia and preferred by millions of local smallholder farmers. It also gained recognition as a food crop in other parts of the world very recently due to its gluten-free grains and its nutritive value. Lodging is the major factor which greatly reduces both yields and quality of tef grain as well as the straw. The current study was conducted to group the lines as their similarities and assess the magnitude of genetic distances among them; then identify the contribution of individual traits for total variations. A total of 49 lines were evaluated for 16 traits using simple lattice design at Holetta and Debre Zeit in 2017 main rainy season. All the traits evaluated over the locations showed highly significant differences among the lines except fertile tiller per plant, while the lines x location interaction effect was highly significant for most of the traits evaluated. Cluster analysis grouped the lines into four clusters based on their similarity. The highest inter-cluster distance noted between clusters two and four while the lowest was between clusters one and four. Principal component analysis showed that about 77.6% of the gross variance among lines explained by five Principal components with eigenvalues greater than unity. This study revealed that four recombinant inbred lines had higher yield than local and standard checks. RIL# 14 showed highest grain yield, low lodging index and other desirable traits than all lines, which could base and strengthen future tef breeding if incorporated as plant material; especially for lodging problem.
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