铬对狼尾草基本生长因子的影响。

N. Joshi, P. Menon
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摘要

农业生产的主要问题之一是重金属污染物。工业化造成了农业土壤和生态系统的重金属污染。金属是地球的自然组成部分,当它们的浓度从自然水平增加时,就会发生生态恶化。本研究通过移植试验,评价了铬污染土壤对狼尾草(Pennisetum glaucum L.)的影响。在培养皿中生长的种子分别暴露于浓度为1、3、5、10、50、100、200、300、500 ppm的铬环境中。每个治疗在随机设计中重复,观察时间为7天。以发芽率、种子活力指数、胚根长度、胚芽长度和鲜重为对照,研究了幼苗对蒸馏水萌发种子的反应。五种不同的铬浓度,即5、10、50、100和200 ppm,应用于植物。每个治疗均采用随机设计,观察时间为45天。以蒸馏水为对照,研究了各植株的根长、茎长、鲜重、总叶绿素含量、蛋白质含量和重金属含量。根长和茎长随移栽Cr浓度的增加而减小。随着Cr水平的增加,所选参数逐渐降低。这些值与根和芽组织中铬的植物积累增加密切相关。观察到,铬对所有参数的有害影响与所采用溶液的浓度成正比,从50 ppm起对生长的抑制作用更为明显。狼尾草作为一种可食用作物,虽然在植物修复技术中具有很好的应用潜力,但它不能用于超积累铬来去除土壤中的铬。
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Effect of chromium on basic growth factors of Pennisetum glaucum L.
One of the main concerns of agricultural production is heavy metal pollutants. The industrialization has resulted in the heavy metal contamination of agricultural soil and ecosystems. Metals are a natural component of the earth,  it is when their concentration increases from natural levels, ecological deterioration occurs. In the present study, transplant experiments were conducted to evaluate the effect of chromium-contaminated soil in Pennisetum glaucum L. The seeds growing in petridishes were exposed to chromium, in increasing concentrations of 1, 3, 5, 10, 50, 100, 200, 300, 500 ppm. Each treatment was replicated in a randomized design and observed over a period of 7 days. The seedlings were studied for their response based on germination rate, seed vigour index, length of the radicle, length of plumule, and fresh weight against seeds germinated using distilled water as a control. Five different chromium concentrations i.e., 5, 10, 50, 100 & 200 ppm, were applied to the plants. Each treatment was replicated in a randomized design and observed for 45 days. The plants were studied for the length of root, length of shoot, fresh weight, total chlorophyll content, protein content, and heavy metal analysis compared to a set irrigated using distilled water as a control. The root and shoot lengths decreased with an increase in Cr concentrations in the transplants. A gradual decrease was observed in the selected parameters, with an increase in Cr levels. The values related well with increased Phyto-accumulation of chromium within the tissues of both roots and shoots. It was observed that chromium's harmful effects on all the parameters were directly proportional to the concentration of solution employed, with the inhibition of growth being more pronounced from 50 ppm onwards. As Pennisetum glaucum L. an edible crop despite showing a good potential for application in phytoremediation techniques, it can’t be used to hyper accumulate chromium to remove it from the soil.
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