Production of BartonellaGenus-Specific Monoclonal Antibodies

ABSTRACT Monoclonal antibodies (MAbs) which react with heat-resistant proteins with molecular masses of 32 to 33 kDa of 14 differentBartonella species were produced. These antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except MAb B3D4, which reacted withChlamydia psittaci and Chlamydia trachomatis at low titers. The identification of a common Bartonellaantigenic protein will make it possible to later produce a diagnostic antigen by cloning and expressing it in Escherichia coli. Moreover, these MAbs allow all Bartonella species to be identified to the genus level.