药用植物(印楝)用作饲料和民族兽医实践的生殖效应:来自动物模型的新见解

Challenges Pub Date : 2022-08-13 DOI:10.3390/challe13020040
Ugochinyere J. Njoga, I. Jaja, Osita S. Onwuka, S. Ilo, I. Eke, K. O. Abah, C. Oguejiofor, I. Ochiogu
{"title":"药用植物(印楝)用作饲料和民族兽医实践的生殖效应:来自动物模型的新见解","authors":"Ugochinyere J. Njoga, I. Jaja, Osita S. Onwuka, S. Ilo, I. Eke, K. O. Abah, C. Oguejiofor, I. Ochiogu","doi":"10.3390/challe13020040","DOIUrl":null,"url":null,"abstract":"In some African and Asian countries, Azadirachta indica (AI) has been fed to livestock for decades and traditionally used to treat certain animal and human diseases. Recently, there are suspicions that the plant may possess anti-reproductive properties and concerns that the continued use of AI as forage or for folkloric medicine may detrimentally affect reproduction in the subjects. To address these challenges, this work determined the reproductive and fertility effects of a methanolic seed extract of AI (MSEAI) using adult female albino rats (AFARs) as an experimental model. Sixty-four AFARs were randomly assigned into four groups (A–D) of sixteen rats each. Group A was the control while groups B, C and D were treated daily with 50, 100 and 200 mg/kg of MSEAI respectively, for 28 consecutive days via oral gavage. Blood samples were collected for hormonal and biochemistry assays. Ovarian samples from the experimental rats were harvested for histopathological studies. Thereafter, the remaining experimental rats were bred, and certain fertility indices determined. The mean serum FSH and LH levels were significantly decreased (p ≤ 0.05) in the 100 and 200 mg/kg groups. The histopathological studies revealed massive follicular degeneration in the 100 and 200 mg/kg treatment groups. The fertility indices indicated that the post-implantation survival index was 100% in the control and 0% in the 200 mg/kg treatment group. No abortion occurred in the control and 50 mg/kg groups, but 25% and 100% of the pregnant does aborted in the 100 and 200 mg/kg treatment groups, respectively. Considering that high doses (100 mg/kg and 200 mg/kg) of MSEAI had significant anti-reproductive and antifertility properties, the use of AI as forage or for ethnoveterinary medicine in breeding females may adversely affect their reproductive potentials. However, the anti-reproductive and antifertility effects could be utilized in rodent depopulation programs in animal agriculture and as a contraceptive to limit the proliferation of stray dogs, known to be reservoirs of the rabies virus in developing countries. Moreover, the MSEAI could be further refined for human use as an effective, cheap, eco-friendly and acceptable alternative to synthetic/modern contraceptives, the use of which is limited in developing nations due to superstitious beliefs and their multiple side effects.","PeriodicalId":91008,"journal":{"name":"Challenges","volume":"19 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Reproductive Effects of Medicinal Plant (Azadirachta indica) Used as Forage and for Ethnoveterinary Practices: New Insights from Animal Models\",\"authors\":\"Ugochinyere J. Njoga, I. Jaja, Osita S. Onwuka, S. Ilo, I. Eke, K. O. Abah, C. Oguejiofor, I. Ochiogu\",\"doi\":\"10.3390/challe13020040\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In some African and Asian countries, Azadirachta indica (AI) has been fed to livestock for decades and traditionally used to treat certain animal and human diseases. Recently, there are suspicions that the plant may possess anti-reproductive properties and concerns that the continued use of AI as forage or for folkloric medicine may detrimentally affect reproduction in the subjects. To address these challenges, this work determined the reproductive and fertility effects of a methanolic seed extract of AI (MSEAI) using adult female albino rats (AFARs) as an experimental model. Sixty-four AFARs were randomly assigned into four groups (A–D) of sixteen rats each. Group A was the control while groups B, C and D were treated daily with 50, 100 and 200 mg/kg of MSEAI respectively, for 28 consecutive days via oral gavage. Blood samples were collected for hormonal and biochemistry assays. Ovarian samples from the experimental rats were harvested for histopathological studies. Thereafter, the remaining experimental rats were bred, and certain fertility indices determined. The mean serum FSH and LH levels were significantly decreased (p ≤ 0.05) in the 100 and 200 mg/kg groups. The histopathological studies revealed massive follicular degeneration in the 100 and 200 mg/kg treatment groups. The fertility indices indicated that the post-implantation survival index was 100% in the control and 0% in the 200 mg/kg treatment group. No abortion occurred in the control and 50 mg/kg groups, but 25% and 100% of the pregnant does aborted in the 100 and 200 mg/kg treatment groups, respectively. Considering that high doses (100 mg/kg and 200 mg/kg) of MSEAI had significant anti-reproductive and antifertility properties, the use of AI as forage or for ethnoveterinary medicine in breeding females may adversely affect their reproductive potentials. However, the anti-reproductive and antifertility effects could be utilized in rodent depopulation programs in animal agriculture and as a contraceptive to limit the proliferation of stray dogs, known to be reservoirs of the rabies virus in developing countries. Moreover, the MSEAI could be further refined for human use as an effective, cheap, eco-friendly and acceptable alternative to synthetic/modern contraceptives, the use of which is limited in developing nations due to superstitious beliefs and their multiple side effects.\",\"PeriodicalId\":91008,\"journal\":{\"name\":\"Challenges\",\"volume\":\"19 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-08-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Challenges\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/challe13020040\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Challenges","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/challe13020040","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

摘要

在一些非洲和亚洲国家,印度印楝(AI)几十年来一直被用来喂养牲畜,传统上用于治疗某些动物和人类疾病。最近,有人怀疑这种植物可能具有抗生殖特性,并担心继续将其用作饲料或民间药物可能会对受试者的生殖产生不利影响。为了解决这些挑战,本研究以成年雌性白化大鼠(AFARs)为实验模型,确定了AI甲醇种子提取物(MSEAI)对生殖和生育的影响。64只afar随机分为4组(A-D),每组16只。A组为对照组,B、C、D组分别给予50、100、200 mg/kg MSEAI每日灌胃,连续28 D。采集血样进行激素和生化分析。收集实验大鼠卵巢标本进行组织病理学研究。然后,对剩余的实验大鼠进行饲养,测定一定的生育指标。100和200 mg/kg组血清FSH和LH水平显著降低(p≤0.05)。组织病理学研究显示100和200 mg/kg治疗组有大量卵泡变性。生育指标显示,对照组着床后生存指数为100%,200 mg/kg处理组为0%。对照组和50 mg/kg组未发生流产,100和200 mg/kg组流产率分别为25%和100%。考虑到高剂量(100 mg/kg和200 mg/kg)的MSEAI具有显著的抗生殖和抗生育特性,将其作为饲料或民族兽药用于繁殖雌性可能会对其生殖潜力产生不利影响。然而,抗生殖和抗生育作用可用于动物农业的啮齿动物种群减少计划,并作为一种避孕措施来限制流浪狗的繁殖,在发展中国家,流浪狗是狂犬病病毒的宿主。此外,MSEAI还可以进一步改进,作为一种有效、廉价、环保和可接受的合成/现代避孕药的替代品,供人类使用。由于迷信和多重副作用,合成/现代避孕药在发展中国家的使用受到限制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Reproductive Effects of Medicinal Plant (Azadirachta indica) Used as Forage and for Ethnoveterinary Practices: New Insights from Animal Models
In some African and Asian countries, Azadirachta indica (AI) has been fed to livestock for decades and traditionally used to treat certain animal and human diseases. Recently, there are suspicions that the plant may possess anti-reproductive properties and concerns that the continued use of AI as forage or for folkloric medicine may detrimentally affect reproduction in the subjects. To address these challenges, this work determined the reproductive and fertility effects of a methanolic seed extract of AI (MSEAI) using adult female albino rats (AFARs) as an experimental model. Sixty-four AFARs were randomly assigned into four groups (A–D) of sixteen rats each. Group A was the control while groups B, C and D were treated daily with 50, 100 and 200 mg/kg of MSEAI respectively, for 28 consecutive days via oral gavage. Blood samples were collected for hormonal and biochemistry assays. Ovarian samples from the experimental rats were harvested for histopathological studies. Thereafter, the remaining experimental rats were bred, and certain fertility indices determined. The mean serum FSH and LH levels were significantly decreased (p ≤ 0.05) in the 100 and 200 mg/kg groups. The histopathological studies revealed massive follicular degeneration in the 100 and 200 mg/kg treatment groups. The fertility indices indicated that the post-implantation survival index was 100% in the control and 0% in the 200 mg/kg treatment group. No abortion occurred in the control and 50 mg/kg groups, but 25% and 100% of the pregnant does aborted in the 100 and 200 mg/kg treatment groups, respectively. Considering that high doses (100 mg/kg and 200 mg/kg) of MSEAI had significant anti-reproductive and antifertility properties, the use of AI as forage or for ethnoveterinary medicine in breeding females may adversely affect their reproductive potentials. However, the anti-reproductive and antifertility effects could be utilized in rodent depopulation programs in animal agriculture and as a contraceptive to limit the proliferation of stray dogs, known to be reservoirs of the rabies virus in developing countries. Moreover, the MSEAI could be further refined for human use as an effective, cheap, eco-friendly and acceptable alternative to synthetic/modern contraceptives, the use of which is limited in developing nations due to superstitious beliefs and their multiple side effects.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
审稿时长
11 weeks
期刊最新文献
Safe Space for Dialogue—A Practice for Connected Consciousness and Compassion From Life-Skills Research and Training to Sustainability: A Case Study from a Spanish University Supporting Ageing Populations in Developing Countries: A Comparative Analysis of Pension Schemes and Policy Insights A New Vision for Challenges: A Transdisciplinary Journal Promoting Planetary Health and Flourishing for All Unlocking the Transformative Potential of Outdoor Office Work—A Constructivist Grounded Theory Study
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1