Determination of the Povidone-Iodine Contents by High-performance Liquid Chromatography

The povidone-iodine contents in liquid and ointment were determined using high-performance liquid chromatography (HPLC). The chromatograph equipped reversed phase ODS column and detector was set at 355 nm. The flow rate of the mobile phase of methanol: water (7: 3) containing 1% KI was 1.0ml/min.The retention time of povidone-iodine was 3.9 min. When this HPLC was applied to the povidone-iodine preparations (gurgle and solution) on the commercial market, the povidoneiodinecontents were found to comprise more than 95%. In the povidone-iodine sugar ointment, which was prepared in the hospital pharmacy, admixed with lysozyme chloride ointment, the iodine contents by this HPLC had a good correlation with the available iodine contents by titration (r=0.9999). Furthermore, this method could also be applied to povidoneiodine in the organic solution after extracting the povidone-iodine sugar ointment admixed with lysozyme chloride ointment.