部分药用植物重金属测定及植物化学分析

S. Gunavathy, H. B. Sherine
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摘要

-数千年来,许多文化和传统都在使用天然产品。植物合成大量的次生代谢产物(植物化合物)并在其中积累。生物碱、黄酮类化合物、酚类化合物、类固醇和香豆素等可能具有生物活性的植物化合物是药物发现的可能来源。进行了一项研究,分析了首选药用植物中微量必需和非必需重金属的存在。原子吸收光谱法测定了所选药用植物中重金属的含量。本研究结果表明,所分析的药材中含有重金属(Fe、Cu、Mn、Zn、Ni、Co、Pb、Al、V、Cr、Mo、Hg、As和Cd)。采用原子吸收分光光度法测定了三种植物提取物中重金属的浓度(ppm)。定性分析表明,大部分水合醇叶提取物的主要成分为生物碱。三种乙醇叶提取物均含有总酚、类黄酮、甾体、生物碱和单宁。总酚和生物碱在叶样品中含量较高,根据规定的允许限量对结果进行判断,表明中药中重金属含量在食用中药材的允许限量范围内。本研究的目的是确定所选草药植物中的重金属含量,并强调与重金属毒性水平存在相关的健康问题。大量的植物化学成分的存在表明罗勒叶植物具有较高的药用价值,可以广泛研究提取对人类有益的天然化合物,而不是使用有副作用的合成药物来实现更高的商业化生产。
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Determination of heavy metals and phytochemical analysis of some selected medicinal plants
- Natural products had been essentially used by many cultures and traditions from thousands of years. Plants synthesize massive varieties of substances called secondary metabolities (Phytocompounds) and accumulated in it. The possible bioactive phytocompounds like alkaloids, flavanoids, and phenolic compounds, steroids and coumarins etc., are possible sources for drug discovery. A study was conducted to analyse the presence of traces of essential and non essential heavy metals in the preferred medicinal plants. AAS is used to examine the presence of heavy metals in the selected medicinal plant. Results obtained in the present study showed that the medicinal herb analysed contain heavy metals (Fe, Cu, Mn, Zn, Ni, Co, Pb, Al, V, Cr, Mo, Hg, As and Cd). The concentration (ppm) of heavy metals in the plant extracts like Plectranthus mollis, Elaeagnus conferta and Grewia tilaefolia leaf extracts were found using atomic absorption spectrophotometer. The qualitative analysis showed that alkaloids were mainly seen in most of the samples of Hydro alcoholic leaf extracts. Total phenol, Flavonoid, Steroids, Alkaloid and Tannins were present in all the three samples extracted by Hydro alcoholic leaf extracts. Total phenol and Alkaloid were seen more in leaf samples, from the judgment of the results with the defined permissible limits, it was accomplished that the levels of heavy metals present in the herb falls in the permissible limits for consumed medicinal herbs. Purpose of this study is to determine heavy metals contents in chosen herbal plants and also to highlight the health concerns related to the presence of toxic levels of heavy metals. The presence of high amount of phytochemical compounds put forward that the Grewia tilaefolia plant has higher medicinal value and can be extensively studied to extract the natural compounds which are beneficial to human beings and that could be commercialized for higher production than using synthetic drugs with side effects.
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