杂交松树种群中亲本等位基因非对称保留的功能意义

IF 3.7 1区 生物学 Q1 Agricultural and Biological Sciences Journal of Systematics and Evolution Pub Date : 2023-03-10 DOI:10.1111/jse.12953
Chang Qu, Hong-Na Kao, Hui Xu, Bao-Sheng Wang, Zhi-Ling Yang, Qi Yang, Gui-Feng Liu, Xiao-Ru Wang, Yan-Jing Liu, Qing-Yin Zeng
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摘要

杂交基因组通常含有不对称的亲本贡献。然而,要推断针叶树杂交种群中亲本等位基因的非对称保留的功能意义却很困难。在这里,我们研究了杂交松树 Pinus densata 及其亲本(Pinus tabuliformis 和 Pinus yunnanensis)中谷胱甘肽 S-转移酶(GST)基因家族的多样性。植物 GST 在保护植物免受生物和非生物胁迫方面发挥着重要作用。本研究从这三个物种中鉴定并克隆了 19 个直向同源的 GST 基因组。我们检测了它们在不同组织中的表达,然后纯化了相应的蛋白质,以鉴定它们的酶活性和对不同底物的特异性。我们发现,在 19 个 GST 同源群中,基因表达和对不同底物的酶活性普遍存在差异。在 19 个基因位点中,P. densata 有 17 个基因位点优先保留了类似云南虫草的 GST。我们首次测定了针叶树种的 GST 晶体结构,分辨率为 2.19 Å。在此结构基础上,我们进行了定点突变,替换了不同野生型 GSTs 中的残余氨基酸,以了解它们对功能的影响。在 P. densata 和 P. tabuliformis 的原生 GSTs 中,氨基酸残基的互补替换显示了酶功能的显著变化,并确定了控制 GSTs 活性的关键位点。这项研究为评估针叶树基因组序列变异的功能意义提供了一种方法。我们的研究还揭示了控制 P. densata 基因组中亲本等位基因选择性保留的合理机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Functional significance of asymmetrical retention of parental alleles in a hybrid pine species complex

Hybrid genomes usually harbor asymmetrical parental contributions. However, it is challenging to infer the functional significance of asymmetrical retention of parental alleles in hybrid populations of conifer trees. Here we investigated the diversity in the glutathione S-transferase (GST) gene family in a hybrid pine Pinus densata and its parents (Pinus tabuliformis and Pinus yunnanensis). Plant GSTs play major roles in protecting plants against biotic and abiotic stresses. In this study, 19 orthologous groups of GST genes were identified and cloned from these three species. We examined their expression in different tissues, and then purified the corresponding proteins to characterize their enzymatic activities and specificities toward different substrates. We found that among the 19 GST orthologous groups, divergence in gene expression and in enzymatic activities toward different substrates was prevalent. P. densata preferentially retained P. yunnanensis-like GSTs for 17 out of the 19 gene loci. We determined the first GST crystal structure from conifer species at a resolution of 2.19 Å. Based on this structure, we performed site-directed mutagenesis to replace amino acid residuals in different wild-types of GSTs to understand their functional impacts. Reciprocal replacement of amino acid residuals in native GSTs of P. densata and P. tabuliformis demonstrated significant changes in enzyme functions and identified key sites controlling GSTs activities. This study illustrates an approach to evaluating the functional significance of sequence variations in conifer genomes. Our study also sheds light on plausible mechanisms for controlling the selective retention of parental alleles in the P. densata genome.

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来源期刊
Journal of Systematics and Evolution
Journal of Systematics and Evolution Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
7.40
自引率
8.10%
发文量
1368
审稿时长
6-12 weeks
期刊介绍: Journal of Systematics and Evolution (JSE, since 2008; formerly Acta Phytotaxonomica Sinica) is a plant-based international journal newly dedicated to the description and understanding of the biological diversity. It covers: description of new taxa, monographic revision, phylogenetics, molecular evolution and genome evolution, evolutionary developmental biology, evolutionary ecology, population biology, conservation biology, biogeography, paleobiology, evolutionary theories, and related subjects.
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