DEVELOPMENT OF LEVOFLOXACIN LOADED MICROEMULSION FORMULATED WITH MUSTARD OIL

The aim of the current study is to develop Levofloxacin loaded microemulsion formulated with mustard oil for better skin penetration effect and to obtain combined effect of both the oil and the drug to improve the efficacy with a reduced dose. Primarily we developed a pseudo ternary phase diagrams to find out the region of microemulsion formation zone by using oil (mustard oil), surfactant (Tween 20 and Tween 80), and co-surfactant (propylene glycol) by water titration method. We formulated six different formulations (MM1-MM6) by varying the concentrations of the oil water and surfactant and cosurfactant ratio. The developed microemulsion formulation was characterized for various parameters % Transmittance, viscosity, pH, drug content, surface morphology, zeta potential, and in-vitro drug release study. The selected microemulsion formulation is further converted in to microemulgel by dispersing the ME into 2%w/W Carbopol gel (MM-G) and various parameters are evaluated for the gel. The antimicrobial efficacy was carried out for ME and Microemulgel by well diffusion method against Staphylococcus aureus (MTCC: 737) compared with the standard streptomycin which showed that MM3 and MM-G have a better antimicrobial effect than standard proved that the drug levofloxacin and the mustard oil shows the synergistic effect in the Microemulsion formulation with better skin penetration effect.