石竹叶不同溶剂提取物的植物化学性质

O. Popoola
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引用次数: 3

摘要

在提取药用植物中急需的生物活性物质的过程中,选择合适的溶剂是至关重要的。本研究评估了四种溶剂(甲醇、己烷、乙酸乙酯和水)提取物的植物化学状态,将植物叶片用溶剂冷浸3天;采用标准方法对皂苷、生物碱、单宁、心苷、黄酮类酚类和萜类进行筛选和定量测定。植物化学筛选表明甲醇提取物中存在所有植物化合物,而其他提取物中不同植物化学物质的可用性存在差异,定量结果显示生物碱为1.79 ~ 18.90 mg/100g,黄酮类化合物为2.54 ~ 10.53 mg/100g,皂苷为2.00 ~ 12.23 mg/100g,单宁为ND-4.83 mg/100g,苯酚为ND-6.65 mg/100g,心苷为ND-2.08 mg/100g,萜类化合物为ND-1.98 mg/100g;不同溶剂对植物化学物质的提取效果差异显著(p<0.05),其中甲醇和水提液对植物化学物质的提取效果最好。因此,用甲醇和水两种方法均可获得最佳的提取方法。
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Phytochemical Status of Different Solvent Extracts of Anthocleista djalonensis A. Chev Leaves
The choice of suitable solvent is essential in the extraction of much needed bioactive phytochemicals present in medicinal plants. This study assessed the phytochemical status of four solvents (methanol, hexane, ethylacetate and aqueous) extracts of Anthocleista djalonensis, leaves of the plants were extracted with the solvents by cold maceration for three days; screening and quantitative determination of saponin, alkaloid, tannin, cardiac glycosides, flavonoids phenols and terpenoids was done using standard methods. Phytochemical screening indicates the presence of all the phytocompound in the methanol extract, while other extracts shows variation in the availability of different phytochemicals, quantitative results shows alkaloid ranged from 1.79-18.90 mg/100g, flavonoids: 2.54-10.53 mg/100g, saponin: 2.00-12.23 mg/100g, tannin: ND-4.83 mg/100g, phenol: ND-6.65 mg/100g, cardiac glycoside: ND-2.08 mg/100g, and terpenoids: ND-1.98 mg/100g; significant difference (p<0.05) exist in each phytochemicals across the solvents where methanol and the aqueous extracts gave the best extraction efficiency for the phytochemicals. Consequently, the optimum extraction of useful phytochemicals from A. djalonensis could be achieved using either methanol and water.
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