简单比色法测定响应面法优化聚苯乙烯生物降解

IF 0.4 Q4 ENGINEERING, ELECTRICAL & ELECTRONIC EMITTER-International Journal of Engineering Technology Pub Date : 2019-12-24 DOI:10.14419/ijet.v7i4.14.27567
T. K. Meng, D. Y.Y. Beng, A. S. Mohd Kassim, A. H. A. Razak, N. A. Mohd Fauzi
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引用次数: 1

摘要

马来西亚每年丢弃近28万吨聚苯乙烯(PS)废物。禁止使用PS食品包装旨在最大限度地减少这种“白色污染”。然而,尽管停止生产PS,现有的PS废物量仍保持不变。因此,本研究利用分离菌株aryabhattai作为生物降解难降解塑料的替代方案,对PS的生物降解进行了优化研究。pH和温度作为自变量分别在三个水平(pH: 6、7.5和9,温度:25°C、35°C和45°C)下通过三个水平因子进行研究,并使用Design Expert Version 10.0使用响应面法(RSM)进行分析。采用简单比色法测定了aryabhattai芽孢杆菌的生物降解活性。将aryabhattai芽孢杆菌接种于ps染色膜上,并在设计的条件下进行培养。作为生物降解的结果,捕获的亚甲基蓝染料被释放出来并进行光度测定。在pH为9、温度为25℃的条件下,蓝色染料吸光度的最佳读数为75.9%。该研究为今后研究aryabhattai的生物降解活性提供了重要信息,可用于废物管理。
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Optimization of Polystyrene Biodegradation using Response Surface Methodology (RSM) Measured by Simple Colorimetric Method
Nearly 280 kilotons of polystyrene (PS) waste being discarded yearly in Malaysia. Banning usage of PS food packaging aims to minimize this “white pollution”. However, the existing PS waste volume remains the same despite the termination of PS production. Therefore, this study has investigated the optimization of biodegradation of PS using isolated strain Bacillus aryabhattai as an alternative solution to the biodegradation of recalcitrant plastic. The effect of pH and temperature as independent variables were studied at three levels each (pH: 6, 7.5 and 9, temperature: 25 °C, 35 °C and 45 °C) under the designation by three level-factorial and analysed using response surface methodology (RSM) by Design Expert Version 10.0. The biodegradation activity of Bacillus aryabhattai was quantified using simple colorimetric method. Bacillus aryabhattai was inoculated on the dye PS-film and incubated under designed conditions. As a result of biodegradation, the entrapped methylene blue dye was released and measured photometrically. Optimum conditions for the highest reading of blue dye absorbance were obtained at pH 9 and temperature of 25ºC with the desirability of 75.9%. This study provides significant information on the biodegradation activity of Bacillus aryabhattai for future research that can be applied in waste management. 
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来源期刊
EMITTER-International Journal of Engineering Technology
EMITTER-International Journal of Engineering Technology ENGINEERING, ELECTRICAL & ELECTRONIC-
自引率
0.00%
发文量
7
审稿时长
12 weeks
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