β-氨基丁酸对拟南芥(十字花科)叶片花色苷的影响

Yan Du, Di-qiu Yu
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引用次数: 0

摘要

为分析β-氨基丁酸(BABA)对拟南芥叶片花青素含量的影响,在30株老株上喷施BABA,对照浇水。经BABA处理后,花青素含量显著低于对照。RT-PCR结果显示,CHS、LDOX、UF3GT较对照下调,PAL则相反。与此同时,在花青素降解过程中起重要作用的PPO活性明显高于对照。此外,BABA处理还降低了叶片的抗氧化能力、细胞死亡率和电导率。结果表明,BABA可能抑制拟南芥叶片花青素的积累。
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Effect of β-aminobutyrie acid on anthocyanin of leaves of Arabidopsis thaliana (Cruciferae).
To analyze the effect of β-aminobutyrie acid (BABA) on anthocyanin of leaves of Arabidopsis,30-old plants were sprayed with BABA while the control were sprayed with water. After treated with BABA,the content of anthocyanin was significantly lower than that of control. Furthermore,the results from RT-PCR showed that CHS,LDOX,UF3GT were down regulated compared with contro1,while PAL showed an opposite trend. At the same time,the activity of PPO,which played an important role in the degradation of anthocyanin,showed higher level than control. In addition,the antioxidant capacity,the death rate of cells and electrical conductivity of leaves were also decreased with BABA treatment. All results suggested that BABA might inhibit the accumulation of anthocyanin in leaves of Arabidopsis in vitro.
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