Stringent isolator technology when handling highly pathogenic BSL-4 materials

A BSL-4 laboratory is extremely isolated, in negative pressureoften located in a separate building or in an isolated and restricted zone of the building. The laboratory also features a dedicated supply and exhausts air, as well as protected vacuum lines and reproducible decontamination systems. What alternative with the same separation technologies, knowing that each piece of technology has to be validated prior and after use.