{"title":"榕树内生病原菌Phomopsis sp.抗菌代谢物的分离与鉴定。(桑科)","authors":"Devaraju Rakshith , Parthasarathy Santosh , Sreedharamurthy Satish","doi":"10.1016/j.ijcas.2013.08.006","DOIUrl":null,"url":null,"abstract":"<div><h3>Background/Aim</h3><p>An endophytic fungus belongs to the genus <em>Phomopsis</em> was isolated from stem of <em>Ficus pumila</em> (Moraceae) and identified based on morphological and molecular characteristics.</p></div><div><h3>Methods</h3><p>Antimicrobial potential was investigated by agar plug, disc diffusion and TLC bioautography agar overlay methods. Detection of polyketide synthase (PKS) genes using DOP-PCR techniques.</p></div><div><h3>Results</h3><p>Endophytic <em>Phomopsis</em> sp. identified by morphological as well as molecular techniques, which exhibited broad a spectrum antimicrobial activity against Gram positive and Gram negative human and phytopathogenic bacteria and fungi. TLC bioautography detects the production of antimicrobial metabolites.</p></div><div><h3>Conclusion</h3><p>This preliminary study revealed that endophytic <em>Phomopsis</em> species are prolific producer of antimicrobial metabolites with wide range of antimicrobial activity.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 3","pages":"Pages 156-160"},"PeriodicalIF":0.0000,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.08.006","citationCount":"24","resultStr":"{\"title\":\"Isolation and characterization of antimicrobial metabolite producing endophytic Phomopsis sp. from Ficus pumila Linn. (Moraceae)\",\"authors\":\"Devaraju Rakshith , Parthasarathy Santosh , Sreedharamurthy Satish\",\"doi\":\"10.1016/j.ijcas.2013.08.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background/Aim</h3><p>An endophytic fungus belongs to the genus <em>Phomopsis</em> was isolated from stem of <em>Ficus pumila</em> (Moraceae) and identified based on morphological and molecular characteristics.</p></div><div><h3>Methods</h3><p>Antimicrobial potential was investigated by agar plug, disc diffusion and TLC bioautography agar overlay methods. Detection of polyketide synthase (PKS) genes using DOP-PCR techniques.</p></div><div><h3>Results</h3><p>Endophytic <em>Phomopsis</em> sp. identified by morphological as well as molecular techniques, which exhibited broad a spectrum antimicrobial activity against Gram positive and Gram negative human and phytopathogenic bacteria and fungi. TLC bioautography detects the production of antimicrobial metabolites.</p></div><div><h3>Conclusion</h3><p>This preliminary study revealed that endophytic <em>Phomopsis</em> species are prolific producer of antimicrobial metabolites with wide range of antimicrobial activity.</p></div>\",\"PeriodicalId\":100693,\"journal\":{\"name\":\"International Journal of Chemical and Analytical Science\",\"volume\":\"4 3\",\"pages\":\"Pages 156-160\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.08.006\",\"citationCount\":\"24\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Chemical and Analytical Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0976120913000442\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Chemical and Analytical Science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0976120913000442","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation and characterization of antimicrobial metabolite producing endophytic Phomopsis sp. from Ficus pumila Linn. (Moraceae)
Background/Aim
An endophytic fungus belongs to the genus Phomopsis was isolated from stem of Ficus pumila (Moraceae) and identified based on morphological and molecular characteristics.
Methods
Antimicrobial potential was investigated by agar plug, disc diffusion and TLC bioautography agar overlay methods. Detection of polyketide synthase (PKS) genes using DOP-PCR techniques.
Results
Endophytic Phomopsis sp. identified by morphological as well as molecular techniques, which exhibited broad a spectrum antimicrobial activity against Gram positive and Gram negative human and phytopathogenic bacteria and fungi. TLC bioautography detects the production of antimicrobial metabolites.
Conclusion
This preliminary study revealed that endophytic Phomopsis species are prolific producer of antimicrobial metabolites with wide range of antimicrobial activity.
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