基于配体-受体相互作用的无标记电化学生物传感器

2018 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) Pub Date : 2018-08-01 DOI:10.1109/3M-NANO.2018.8552168

Xiyuan Liu, Ru Zhang, C. Rejeeth, Sohel Rana, D. Gurav, Kun Qian

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引用次数: 0

摘要

我们展示了一种基于其相应的生物活性物质(BS)与细胞膜受体之间的配体-受体相互作用的无标记电化学生物传感器，用于检测细胞表面受体(CSR)。以壳聚糖(Chi)和生物活性物质偶联多壁碳纳米管(MWCNTs-BS)为材料，采用逐层沉积法制备了生物传感器。我们使用设计生物传感器优化的CSR检测方法产生了从1 ng/mL到10,000 ng/mL的线性检测范围，达到了0.106 ng/mL的检测限(LOD)。

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A Label-Free Electrochemical Biosensor Based on Ligand-Receptor Interaction

We demonstrated a label-free electrochemical biosensor for detection of cell surface receptor (CSR) based on ligand-receptor interaction between its corresponding bioactive substance (BS) and the cell membrane receptor. The biosensor was fabricated through layer-by-layer (LBL) deposition method using chitosan (Chi) and bioactive substance conjugated multi-walled carbon nanotubes (MWCNTs-BS). Our optimized CSR detection approach using the designer biosensor yielded a linear detection range wildly from 1 ng/mL to 10,000 ng/mL, achieving the limit of detection (LOD) of 0.106 ng/mL.

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2018 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)

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