必需微量元素对羔羊肌肉组织蛋白蛋白质组学的影响

Q3 Economics, Econometrics and Finance Food Processing: Techniques and Technology Pub Date : 2023-06-23 DOI:10.21603/2074-9414-2023-2-2443
T. Giro, A. Kulikovsky, A. Giro
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引用次数: 0

摘要

蛋白质组学技术使评估肉类原料在不同加工阶段的组成成为可能。羔羊肌肉组织的蛋白质组学研究有助于扩大对必需有机微量元素对羔羊肌肉组织蛋白质相互作用影响的科学认识。研究目的是鉴定和定量生长在添加微量元素饲料添加剂上的羔羊肌肉组织蛋白质。该研究选用了7个月大的Edilbaev品种的幼羊的肉,这些羊在其饮食中添加了Yoddar-Zn和DAFS-25添加剂。试验期105 d。采用原子吸收光谱法测定羔羊肌肉组织的微量元素组成。利用O'Farrell 's二维凝胶电泳(2-DE)等电聚焦双酚啉(IEF-PAGE)对蛋白质组学进行了鉴定。在饲喂饲料添加剂约达-锌和DAFS-25 105 d的羔羊样品中鉴定出铝、碘、硅、硒和锌。主要蛋白片段有8个分子量为12-15 kDa, 42个分子量为16-30 kDa, 45个分子量为35-110 kDa (pI 5.0-8.0)。样品中含有谷胱甘肽- s -转移酶,该酶负责有毒化合物的生物转化,维持细胞内稳态和抗逆性。所有实验样品均含有磷酸三糖异构酶(糖酵解酶)。测试还发现了超氧化物歧化酶,它能将超氧化物自由基催化成过氧化物和氧气,从而保护人体细胞免受自由基的侵害。本研究为创新饲料添加剂对羊肉肌肉组织中发生的分子机制的影响以及对肉蛋白蛋白质组学特征和电泳活性的影响提供了相关数据。结果表明,添加有机微量元素的饲料添加剂是有效的。结果可用于模拟和调整自溶,以获得具有必要工艺性能的肉。
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Effect of Essential Microelements on Proteomic Profile of Lamb Muscle Tissue Protein
Proteomic technologies make it possible to evaluate the composition of meat raw materials at different stages of processing. Proteomic studies of lamb muscle tissue help to expand scientific knowledge about the effect of essential organic microelements on the interaction of lamb muscle tissue proteins. The research objective was to identify and quantify lamb muscle tissue proteins from young sheep grown on feed additives fortified with microelements. The research featured meat from young sheep of the Edilbaev breed aged 7 months that consumed additives Yoddar-Zn and DAFS-25 as part of their diet. The experiment lasted 105 days. The microelement composition of lamb muscle tissue underwent atomic absorption spectrometry. The proteomic profile was identified using O'Farrell’s two-dimensional gel electrophoresis (2-DE) with isoelectrofocusing in ampholine (IEF-PAGE). Aluminum, iodine, silicon, selenium, and zinc were identified in lamb samples from young sheep that received feed additives Yoddar-Zn and DAFS-25 for 105 days. Major protein fractions included eight with a molecular weight of 12–15 kDa, 42 with 16–30 kDa, and 45 with 35–110 kDa (pI 5.0–8.0). The samples contained glutathione-S-transferase, which is responsible for biotransformation of toxic compounds, maintenance of intracellular homeostasis, and stress resistance. All the experimental samples had triose phosphate isomerase (glycolysis enzyme). The tests also revealed superoxide dismutase, which catalyzes the superoxide radical into peroxides and oxygen, thus protecting body cells from free oxygen radicals. The research provided relevant data on the effect of innovative feed additives on the molecular mechanisms that occur in mutton muscle tissue and affect the proteomic profile of meat proteins and electrophoretic activity. The feed additives with organic microelements proved efficient. The results can be used to model and adjust autolysis in order to obtain meat with the necessary technological properties.
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来源期刊
Food Processing: Techniques and Technology
Food Processing: Techniques and Technology Engineering-Industrial and Manufacturing Engineering
CiteScore
1.40
自引率
0.00%
发文量
82
审稿时长
12 weeks
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