{"title":"透明聚酰亚胺薄膜在生物医学工程中作为微结构生物细胞培养片的评价","authors":"Hirotaka Maenosono, H. Saito, Y. Nishioka","doi":"10.1109/OMN.2013.6659088","DOIUrl":null,"url":null,"abstract":"Potentials of the transparent polyimide (PI) substrate were investigated for a possible biological cell culture sheet. Stromal marrow cells (OP9) were used to test the cell culture characteristics, and the OP9 cell culture was compared on three kinds of polymer films of transparent PI, parylene (PA), and polyetheretherketone (PEEK). Microtrenches with the width of 5 μm and the depth of 5 μm were fabricated using hot embossing technique on these films. The cultured cell of OP9 was observed on the transparent PI sheet and PA sheet, but not on PEEK sheet. The culture of OP9 cells were easily observed on the transparent PI sheet using an optical microscope.","PeriodicalId":6334,"journal":{"name":"2013 International Conference on Optical MEMS and Nanophotonics (OMN)","volume":"18 1","pages":"119-120"},"PeriodicalIF":0.0000,"publicationDate":"2013-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Evaluation of transparent polyimide film as biological cell culture sheet with microstructures for biomedical engineering\",\"authors\":\"Hirotaka Maenosono, H. Saito, Y. Nishioka\",\"doi\":\"10.1109/OMN.2013.6659088\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Potentials of the transparent polyimide (PI) substrate were investigated for a possible biological cell culture sheet. Stromal marrow cells (OP9) were used to test the cell culture characteristics, and the OP9 cell culture was compared on three kinds of polymer films of transparent PI, parylene (PA), and polyetheretherketone (PEEK). Microtrenches with the width of 5 μm and the depth of 5 μm were fabricated using hot embossing technique on these films. The cultured cell of OP9 was observed on the transparent PI sheet and PA sheet, but not on PEEK sheet. The culture of OP9 cells were easily observed on the transparent PI sheet using an optical microscope.\",\"PeriodicalId\":6334,\"journal\":{\"name\":\"2013 International Conference on Optical MEMS and Nanophotonics (OMN)\",\"volume\":\"18 1\",\"pages\":\"119-120\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-11-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2013 International Conference on Optical MEMS and Nanophotonics (OMN)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/OMN.2013.6659088\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2013 International Conference on Optical MEMS and Nanophotonics (OMN)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/OMN.2013.6659088","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of transparent polyimide film as biological cell culture sheet with microstructures for biomedical engineering
Potentials of the transparent polyimide (PI) substrate were investigated for a possible biological cell culture sheet. Stromal marrow cells (OP9) were used to test the cell culture characteristics, and the OP9 cell culture was compared on three kinds of polymer films of transparent PI, parylene (PA), and polyetheretherketone (PEEK). Microtrenches with the width of 5 μm and the depth of 5 μm were fabricated using hot embossing technique on these films. The cultured cell of OP9 was observed on the transparent PI sheet and PA sheet, but not on PEEK sheet. The culture of OP9 cells were easily observed on the transparent PI sheet using an optical microscope.
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