{"title":"青蒿蜡菊精油化学成分及对结肠癌细胞的抑菌和细胞毒作用的研究及凋亡基因的PCR表达分析","authors":"امیر میرزایی, سپیده پیروزمند, زهرا موسوی, عبدالحسین روستاییان","doi":"10.29252/qums.12.3.9","DOIUrl":null,"url":null,"abstract":"Methods: In this study, the essential oil of H. artemisioides was collected by hydrodistillation method using Clevenger apparatus. Chemical composition of essential oil was identified using a GC-MS device. Subsequently, antibacterial effect of the essential oil on pathogenic bacteria and its cytotoxic activity on colon cancer cell line (HT29), were determined using MIC and MTT methods, respectively. The expression of Bax and Bcl2 apoptotic genes in HT29 cell line, was assessed using real time PCR method.","PeriodicalId":20865,"journal":{"name":"Qom University of Medical Sciences Journal","volume":"108 1","pages":"9-18"},"PeriodicalIF":0.0000,"publicationDate":"2018-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation of Chemical Composition of Helichrysum artemisioides Essential Oil, and its Antibacterial and Cytotoxic Effects on Colon Cancer Cell Line and Analysis of Apoptotic Gene Expression Using PCR Method\",\"authors\":\"امیر میرزایی, سپیده پیروزمند, زهرا موسوی, عبدالحسین روستاییان\",\"doi\":\"10.29252/qums.12.3.9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Methods: In this study, the essential oil of H. artemisioides was collected by hydrodistillation method using Clevenger apparatus. Chemical composition of essential oil was identified using a GC-MS device. Subsequently, antibacterial effect of the essential oil on pathogenic bacteria and its cytotoxic activity on colon cancer cell line (HT29), were determined using MIC and MTT methods, respectively. The expression of Bax and Bcl2 apoptotic genes in HT29 cell line, was assessed using real time PCR method.\",\"PeriodicalId\":20865,\"journal\":{\"name\":\"Qom University of Medical Sciences Journal\",\"volume\":\"108 1\",\"pages\":\"9-18\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-05-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Qom University of Medical Sciences Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.29252/qums.12.3.9\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Qom University of Medical Sciences Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29252/qums.12.3.9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Investigation of Chemical Composition of Helichrysum artemisioides Essential Oil, and its Antibacterial and Cytotoxic Effects on Colon Cancer Cell Line and Analysis of Apoptotic Gene Expression Using PCR Method
Methods: In this study, the essential oil of H. artemisioides was collected by hydrodistillation method using Clevenger apparatus. Chemical composition of essential oil was identified using a GC-MS device. Subsequently, antibacterial effect of the essential oil on pathogenic bacteria and its cytotoxic activity on colon cancer cell line (HT29), were determined using MIC and MTT methods, respectively. The expression of Bax and Bcl2 apoptotic genes in HT29 cell line, was assessed using real time PCR method.
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