Validation and Development of Metformin obtained from the Extraction of Bougainvillea California Gold Flowers by UV Spectrophotometry, TLC and FTIR

The project was considered to validate and develop an accurate, simple and precise, UV-Visible spectrophotometric, Thin Layer Chromatography (TLC) and Fourier transform Infrared Spectroscopy (FTIR) method for the estimation of Metformin present in extract of Bougainvillea California Gold flowers by using distilled water as solvent at a wavelength of 232 nm. The linearity of the metformin is 10-50 μg/ml with y = 0.0011x - 0.0006 as the regression equation r2 value was 0.9977. Recovery values for Metformin present in Bougainvillea California Gold flowers extract powder is found to 98.45% - 99.16%. The RSD % of intraday precision and intraday precision is 16.67 % and 61.53% respectively. LOQ and LOD values are 0.26 μg/ml and 0.0.08μg/mL respectively. The robustness %RSD values method was 64.38 – 64.66. Rf value of the extract was found to be 0.586 with 60:40 (methanol and water) in TLC. IR interpretation of both bougainvillea California gold flowers extract and Meformin were found to be similar. So the method was simple, precise, exact and economical. This method could be applicable for quantitative determination of the metformin in Bougainvillea California gold flowers extract.