GC–MS and HPTLC analysis of leaf extract of Madhuca longifolia (Koenig) Linn

Objective

To explore the phytochemical constituents present in the leaves of Madhuca longifolia (Koenig) Linn using GC–MS and HPTLC analysis.

Method

The shade-dried leaves of M. longifolia were extracted with ethanol, the concentrated ethanolic extracts were further subjected to GC–MS. The dried powdered leaves were taken in 50% aqueous alcohol and was refluxed, filtered and dried and used for HPTLC analysis.

Results

The fluorescent band (under 254 nm and 366 nm) at R_f 0.67 in mobile phase Toluene:Ethyl acetate:Formic acid (5:4:1) was found and the marker compound Quercetin was quantified. In GC–MS analysis about twenty bioactive compounds were identified that include the presence of phenolic acids, ketones, aldehydes, carbohydrates, heterocyclic compounds and hydrocarbons.

Conclusion

The results of the present study enhance the traditional use of M. longifolia which possesses several known and unknown bioactive compounds, which may be used as an effective source against various diseases.