嗜热菌线索同源基因的克隆与鉴定

Yann Gibert, S. Spada, J. Wall, J. Pembroke
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引用次数: 0

摘要

通过对嗜热热菌基因组文库的筛选,发现了一个新的嗜热暗示基因,该基因同源物在古细菌和哺乳动物属中均高度保守。Hint属于HIT蛋白超家族,该家族包含两大类:Fhit与真核生物的肿瘤抑制有关,而Hint与推测的蛋白激酶C抑制活性有关。在嗜热T.中,321bp基因的总GC含量为67%,在第三核苷酸位置的GC含量为94.4%,不寻常地没有胸腺嘧啶作为摆动碱基。该基因产物是一个小的高度保守的11996Da预测的可溶性细胞质蛋白,为研究热稳定氨基酸取代提供了理想的机会。在这里,我们报告了新的提示序列的特征。
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Cloning and Characterisation of the Hint Homologue of the Thermophile Thermus thermophilus
Screening of a genomic library of the thermophile Thermus thermophilus revealed a novel thermophilic hint gene, homologues of which are highly conserved in genera from archaea to mammals. Hint belongs to the HIT protein super-family, which contains two broad groups, Fhit, associated with tumour suppression in eukaryotes and Hint with putatitive protein kinase C inhibitory activity. In T. thermophilus the 321bp gene has a GC content of 67% overall and 94.4% in the third nucleotide position, with unusually no thymine as a wobble base. The gene product, a small highly conserved 11996Da predicted soluble cytoplasmic protein, offers an ideal opportunity to investigate thermostabilising amino acid substitutions. Here we report on the characterisation of the novel hint sequence.
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