人软骨肉瘤细胞中Akt、STAT-3、NF-κ b和TLR4的蛋白谱:胰岛素信号通路的潜在治疗靶点

S. Akyol, S. Yuksel, M. A. Gulec, Þemsettin Þahin, K. Demircan, O. Akyol, H. Ozyurt
{"title":"人软骨肉瘤细胞中Akt、STAT-3、NF-κ b和TLR4的蛋白谱:胰岛素信号通路的潜在治疗靶点","authors":"S. Akyol, S. Yuksel, M. A. Gulec, Þemsettin Þahin, K. Demircan, O. Akyol, H. Ozyurt","doi":"10.5455/JEIM.170316.OR.149","DOIUrl":null,"url":null,"abstract":"Objectives: Currently there is no effective chemotherapy for chondrosarcoma. Recent studies report that mesenchymal chondrosarcoma is relatively resistant to radiotherapy but sensitive to chemotherapy in some extend. It is unknown whether receptor tyrosine kinase is activated in chondrosarcoma. Potential new systemic treatment targets have been widely investigated. This study aimed to determine insulin-induced phosphorylation rate of signal proteins Akt, STAT-3, NF-κB and TLR4 by using Western Blot technique to find out a possible therapeutic target for human chondrosarcoma. Methods: Human chondrosarcoma cells (OUMS-27) were induced by 10 μmol/mL insulin for 60 min. The first group was untreated control group, while the others were insulin-induced groups for 10, 30, and 60 min by applying same amount of insulin. After the induction periods, cells were harvested and protein extractions were performed. Phosphorylated and unphosphorylated individual protein levels were detected. Results: Both pSTAT-3/STAT-3 and pNF-κB/NF-κB ratios were found to be remarkably increased (almost 3-fold) in 60 min group (almost 3-fold) compared those of controls. Conclusions: The mechanism of insulin action in OUMS-27 chondrosarcoma cell lines and other human chondrosarcomas has not yet been illuminated. According to our findings, STAT-3 and/or NF-κB could be intracellular molecules that transmit the message of insulin to inside of OUMS-27 cells. Especially the fact that the phosphorylated forms of these proteins increase 3 times after 60 minutes of the insulin induction supports our perspective. These signaling molecules might be considered as potential targets of effective chemotherapy alternatives.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"20 1","pages":"6-11"},"PeriodicalIF":0.0000,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Protein profiles of Akt, STAT-3, NF-κB, and TLR4 in human chondrosarcoma cells: Potential therapeutic targets of insulin signaling pathway -\",\"authors\":\"S. Akyol, S. Yuksel, M. A. Gulec, Þemsettin Þahin, K. Demircan, O. Akyol, H. Ozyurt\",\"doi\":\"10.5455/JEIM.170316.OR.149\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objectives: Currently there is no effective chemotherapy for chondrosarcoma. Recent studies report that mesenchymal chondrosarcoma is relatively resistant to radiotherapy but sensitive to chemotherapy in some extend. It is unknown whether receptor tyrosine kinase is activated in chondrosarcoma. Potential new systemic treatment targets have been widely investigated. This study aimed to determine insulin-induced phosphorylation rate of signal proteins Akt, STAT-3, NF-κB and TLR4 by using Western Blot technique to find out a possible therapeutic target for human chondrosarcoma. Methods: Human chondrosarcoma cells (OUMS-27) were induced by 10 μmol/mL insulin for 60 min. The first group was untreated control group, while the others were insulin-induced groups for 10, 30, and 60 min by applying same amount of insulin. After the induction periods, cells were harvested and protein extractions were performed. Phosphorylated and unphosphorylated individual protein levels were detected. Results: Both pSTAT-3/STAT-3 and pNF-κB/NF-κB ratios were found to be remarkably increased (almost 3-fold) in 60 min group (almost 3-fold) compared those of controls. Conclusions: The mechanism of insulin action in OUMS-27 chondrosarcoma cell lines and other human chondrosarcomas has not yet been illuminated. According to our findings, STAT-3 and/or NF-κB could be intracellular molecules that transmit the message of insulin to inside of OUMS-27 cells. Especially the fact that the phosphorylated forms of these proteins increase 3 times after 60 minutes of the insulin induction supports our perspective. These signaling molecules might be considered as potential targets of effective chemotherapy alternatives.\",\"PeriodicalId\":16091,\"journal\":{\"name\":\"Journal of Experimental and Integrative Medicine\",\"volume\":\"20 1\",\"pages\":\"6-11\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Experimental and Integrative Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/JEIM.170316.OR.149\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Experimental and Integrative Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/JEIM.170316.OR.149","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

目的:目前尚无治疗软骨肉瘤的有效化疗方法。近年来的研究报道,间充质软骨肉瘤对放射治疗相对耐药,但在一定程度上对化疗敏感。目前尚不清楚受体酪氨酸激酶是否在软骨肉瘤中被激活。潜在的新的全身治疗靶点已被广泛研究。本研究旨在通过Western Blot技术检测胰岛素诱导的Akt、STAT-3、NF-κB和TLR4信号蛋白磷酸化率,寻找可能治疗人软骨肉瘤的靶点。方法:用10 μmol/mL胰岛素诱导人软骨肉瘤细胞(OUMS-27) 60 min,第一组为未处理对照组,其余为胰岛素诱导组,分别用相同剂量胰岛素诱导10、30、60 min。诱导期结束后,收获细胞,提取蛋白质。检测磷酸化和未磷酸化的个体蛋白水平。结果:与对照组相比,60 min组pSTAT-3/STAT-3和pNF-κB/NF-κB比值均显著升高(近3倍)。结论:胰岛素在OUMS-27软骨肉瘤细胞系和其他人软骨肉瘤中的作用机制尚未阐明。根据我们的研究结果,STAT-3和/或NF-κB可能是将胰岛素信息传递到OUMS-27细胞内部的细胞内分子。特别是这些蛋白的磷酸化形式在胰岛素诱导60分钟后增加了3倍,这一事实支持了我们的观点。这些信号分子可能被认为是有效化疗替代方案的潜在靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Protein profiles of Akt, STAT-3, NF-κB, and TLR4 in human chondrosarcoma cells: Potential therapeutic targets of insulin signaling pathway -
Objectives: Currently there is no effective chemotherapy for chondrosarcoma. Recent studies report that mesenchymal chondrosarcoma is relatively resistant to radiotherapy but sensitive to chemotherapy in some extend. It is unknown whether receptor tyrosine kinase is activated in chondrosarcoma. Potential new systemic treatment targets have been widely investigated. This study aimed to determine insulin-induced phosphorylation rate of signal proteins Akt, STAT-3, NF-κB and TLR4 by using Western Blot technique to find out a possible therapeutic target for human chondrosarcoma. Methods: Human chondrosarcoma cells (OUMS-27) were induced by 10 μmol/mL insulin for 60 min. The first group was untreated control group, while the others were insulin-induced groups for 10, 30, and 60 min by applying same amount of insulin. After the induction periods, cells were harvested and protein extractions were performed. Phosphorylated and unphosphorylated individual protein levels were detected. Results: Both pSTAT-3/STAT-3 and pNF-κB/NF-κB ratios were found to be remarkably increased (almost 3-fold) in 60 min group (almost 3-fold) compared those of controls. Conclusions: The mechanism of insulin action in OUMS-27 chondrosarcoma cell lines and other human chondrosarcomas has not yet been illuminated. According to our findings, STAT-3 and/or NF-κB could be intracellular molecules that transmit the message of insulin to inside of OUMS-27 cells. Especially the fact that the phosphorylated forms of these proteins increase 3 times after 60 minutes of the insulin induction supports our perspective. These signaling molecules might be considered as potential targets of effective chemotherapy alternatives.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
A comparative study of polyherbal Unani formulations in gestational Urinary tract infections Isolated plexiform neurofibroma in a patient with Tuberous Sclerosis-a rare association - Evaluation clinical, electrocardiographic and laboratory response of reperfusion therapy in patients with first acute myocardial infarction In vitro anthelmintic activity of aqueous extract of Crassocephalum crepidioides (Benth.) S. Moore on Haemonchus contortus. (D-Ser2)Oxm[Lys38-γ-glu-PAL] improves hippocampal gene expression and cognition in a mouse model of type 1 diabetes -
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1