{"title":"奥芬达唑对人工饲养的黄颡鱼幼虫血淋巴生化指标的影响","authors":"Serkan Sugeçti, K. Büyükgüzel","doi":"10.7212/ZKUFBD.V8I2.1380","DOIUrl":null,"url":null,"abstract":"Oxfendazole is used as an antihelminthic drug in parasite infections of animals. Oxfendazole was added at different concentrations\n(0, 0.0015, 0.015, 0.15, and 1.5%) to artificial diet and the wax moth Galleria mellonella larvae were reared to 7th stage. The changes\nin metabolic enzymes activity in G. mellonella hemolymph tissue were investigated. It was found that the activities of alanine\naminotransferase (ALT) and aspartate aminotransferase (AST) was increased which are cell damage biomarkers. The highest\nconcentration of this antihelmintic was increased the enzme activity (ALT) from 329 ± 4.33 to 529. 5 ± 3.17 U/L. Gamma glutamyl\ntransferase (GGT) enzyme activity was increased from 0.87 ± 0.1 to 4.32 ± 0.22 U/L. Creatine kinase (CK) and amylase (AMYL)\nactivities were significantly increased in hemolymph of G. mellonella larvae fed with the highest oxfendazole concentration (1.5%) when\ncompared to the control group. Our results indicate that oxfendazole can be used as an insecticide with a well-adjusted concentrations\nbecause of its low acute toxicity to humans and other non-target organisms.","PeriodicalId":17742,"journal":{"name":"Karaelmas Science and Engineering Journal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Effects of Oxfendazole on Some Biochemical Parameters in Hemolymph of Galleria mellonella L. (Lepidoptera: Pyralidae) Larvae Reared on Artificial Diet\",\"authors\":\"Serkan Sugeçti, K. Büyükgüzel\",\"doi\":\"10.7212/ZKUFBD.V8I2.1380\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Oxfendazole is used as an antihelminthic drug in parasite infections of animals. Oxfendazole was added at different concentrations\\n(0, 0.0015, 0.015, 0.15, and 1.5%) to artificial diet and the wax moth Galleria mellonella larvae were reared to 7th stage. The changes\\nin metabolic enzymes activity in G. mellonella hemolymph tissue were investigated. It was found that the activities of alanine\\naminotransferase (ALT) and aspartate aminotransferase (AST) was increased which are cell damage biomarkers. The highest\\nconcentration of this antihelmintic was increased the enzme activity (ALT) from 329 ± 4.33 to 529. 5 ± 3.17 U/L. Gamma glutamyl\\ntransferase (GGT) enzyme activity was increased from 0.87 ± 0.1 to 4.32 ± 0.22 U/L. Creatine kinase (CK) and amylase (AMYL)\\nactivities were significantly increased in hemolymph of G. mellonella larvae fed with the highest oxfendazole concentration (1.5%) when\\ncompared to the control group. Our results indicate that oxfendazole can be used as an insecticide with a well-adjusted concentrations\\nbecause of its low acute toxicity to humans and other non-target organisms.\",\"PeriodicalId\":17742,\"journal\":{\"name\":\"Karaelmas Science and Engineering Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-12-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Karaelmas Science and Engineering Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7212/ZKUFBD.V8I2.1380\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Karaelmas Science and Engineering Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7212/ZKUFBD.V8I2.1380","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
摘要
奥芬达唑是一种用于动物寄生虫感染的抗寄生虫药。在人工饲料中添加不同浓度(0、0.0015、0.015、0.15、1.5%)的奥芬达唑,饲养至7龄黄蜡蛾幼虫。研究了大黄蜂血淋巴组织代谢酶活性的变化。结果发现,作为细胞损伤标志物的谷丙转氨酶(ALT)和天冬氨酸转氨酶(AST)活性升高。最高浓度的抗虫药可使酶活性(ALT)由329±4.33提高到529。5±3.17 u / l。谷氨酰转移酶(GGT)活性由0.87±0.1 U/L提高到4.32±0.22 U/L。饲喂最高浓度(1.5%)奥芬达唑后,血淋巴中肌酸激酶(CK)和淀粉酶(AMYL)活性显著高于对照组。我们的研究结果表明,由于对人类和其他非靶生物的急性毒性低,奥芬达唑可以作为一种浓度调节良好的杀虫剂使用。
Effects of Oxfendazole on Some Biochemical Parameters in Hemolymph of Galleria mellonella L. (Lepidoptera: Pyralidae) Larvae Reared on Artificial Diet
Oxfendazole is used as an antihelminthic drug in parasite infections of animals. Oxfendazole was added at different concentrations
(0, 0.0015, 0.015, 0.15, and 1.5%) to artificial diet and the wax moth Galleria mellonella larvae were reared to 7th stage. The changes
in metabolic enzymes activity in G. mellonella hemolymph tissue were investigated. It was found that the activities of alanine
aminotransferase (ALT) and aspartate aminotransferase (AST) was increased which are cell damage biomarkers. The highest
concentration of this antihelmintic was increased the enzme activity (ALT) from 329 ± 4.33 to 529. 5 ± 3.17 U/L. Gamma glutamyl
transferase (GGT) enzyme activity was increased from 0.87 ± 0.1 to 4.32 ± 0.22 U/L. Creatine kinase (CK) and amylase (AMYL)
activities were significantly increased in hemolymph of G. mellonella larvae fed with the highest oxfendazole concentration (1.5%) when
compared to the control group. Our results indicate that oxfendazole can be used as an insecticide with a well-adjusted concentrations
because of its low acute toxicity to humans and other non-target organisms.