植物抗氧化、抗炎活性研究工藤提取物及其分离物

H. Bu, Sunjoo Lee
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引用次数: 1

摘要

摘要以黄花菊(Isodon inflexus, Thunb) 80%甲醇粗提物、亚组分和亨利素为原料,研究了其抗氧化和抗炎功能。工藤(I. inflexus)奖赏)。通过生物测定法对2,2 -二苯基-1-1-苦苷基(DPPH)自由基清除活性、黄嘌呤氧化酶抑制和超氧化物自由基清除效果进行评价。乙酸乙酯和丁醇组分对超氧化物自由基的清除活性分别为0.9 μg/mL和0.2 μg/mL,均高于别嘌呤醇(2.2 μg/mL)。在抑制RAW 264.7细胞抗炎活性方面,乙酸乙酯部位表现出较强的抑制NO生成的活性,从其亚部位分离的henryin具有剂量依赖性。乙酸乙酯部位和henryin不仅能抑制iNOS、COX-2 mRNA的表达,还能抑制炎症前细胞因子TNF-α、1L-1β、IL-6 mRNA的表达,且呈剂量依赖性。上述结果表明,黄花蓟马的乙酸乙酯部位具有一定的抗氧化活性。工藤作为一种具有抗氧化和抗炎作用的化妆品成分具有相当大的潜力,henryin可以作为功能参考。
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Antioxidation and Anti-inflammation Activity of Isodon inflexus (Thunb.) Kudo Extract and Its Isolated Substance
The functions of anti-oxidation and anti-inflammation were investigated with the crude 80% methanol extract, subfractions and henryin isolated from Isodon inflexus (Thunb.) Kudo (I. inflexus (Thunb.) Kudo). Antioxidative ability was evaluated by bioassays using 2, 2-diphenyl-1-1-picrydrazyl (DPPH) free radical scavenging activity, xanthine oxidase inhibition, and superoxide radical scavenging effects. Ethyl acetate and butanol fractions exhibited free radical scavenging activity on superoxide with IC50 values of 0.9 μg/mL, 0.2 μg/mL, respectively, which were stronger activity than that of allopurinol (2.2 μg/mL) as reference. For the inhibition of anti-inflammatory activity in RAW 264.7 cell, the ethyl acetate fraction showed strong inhibition activity NO production, and henryin isolated from its subfraction reduced the activity in a dose-dependent manner. Ethyl acetate fraction and henryin suppressed not only mRNA expression of iNOS and COX-2, but also the mRNA expression of pre-inflammatory cytokines such as, TNF-α, 1L-1β, IL-6, in a dose-dependent manner. These results suggested that ethyl acetate fraction of I. inflexus (Thunb.) Kudo has considerable potential as a cosmetics ingredient with an antioxidant and anti-inflammatory effects and henryin can be applied as an functional reference.
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