一种保存噬菌体的简便新方法——初步研究

A. Jape
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引用次数: 0

摘要

从污水中分离出寄主大肠杆菌及其噬菌体。将噬菌体感染的宿主大肠杆菌细胞在特定时间间隔内包封在海藻酸盐中共培养,珠粒在不同温度下保存。将微球溶解以释放噬菌体,并通过每月进行斑块测定来评估释放的噬菌体的稳定性。菌斑形成单位数量随着共培养时间的增加而增加,到共培养45min时,菌斑形成单位从106 pfu/ml增加到108 pfu/ml。在宿主细胞内成熟噬菌体的多个拷贝准备就绪的阶段,在细胞裂解开始之前,被感染的宿主细胞被包裹在海藻酸钙中。
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A NEW SIMPLE METHOD TO PRESERVE PHAGE - PRELIMINARY STUDY PRELIMINARY STUDY
The host E. coli and its phage were isolated from sewage water. The phage infected host, E coli cells were encapsulated in alginate at specific time interval of co-cultivation, beads were stored at different temperatures. Beads were solublised to release phages and stability of released phages was evaluated by performing plaque assay at monthly interval. The number of plaque forming units increased with time of co-cultivation and by 45mins of phage-host co-cultivation, count raised from 106 to 108 pfu/ml. The infected host cells were entrapped in calcium alginate at a stage when multiple copies of mature phage are ready within host cell, and well before commencement of cell lysis.
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