Shouvik Chakravarty, K. Sultana, Sumanta Das, Indrani Chandra
{"title":"积雪草次生代谢产物的植物化学分析及抗氧化活性的紫外可见光谱评价","authors":"Shouvik Chakravarty, K. Sultana, Sumanta Das, Indrani Chandra","doi":"10.2174/2211550109999200821155854","DOIUrl":null,"url":null,"abstract":"\n\nCentella asiatica (L.) is an herbaceous, frost-tender perennial plant belonging\nto the Apiaceae family. It is rich in important secondary metabolites such as terpenoids, flavonoids and\nsaponins. It is used as a traditional medicine for skin infection, cough and asthma.\n\n\n\nThe present study was intended at the quantitative and qualitative analysis of its secondary\nmetabolites. Thin Layer Chromatography (TLC) was performed to separate the compounds according\nto their Rf (Retention factor) values.\n\n\n\nThe total phenolic content of ethanol leaf extract was 45.72 ± 6.05 mg GAE/g determined by\nFolin Ciocalteu’s assay. The maximum content of total flavonoids was found in aqueous leaf extract,\n44.62 ± 2.45 mg QE/g. Aqueous extract of leaf and stem showed the highest antioxidant activity with\nDPPH (2,2- diphenyl-1-picrylhydrazyl) scavenging assay (45.66 ± 1.29 & 45.20 ± 3.0, respectively).\n\n\n\nThe present study revealed the antioxidant properties in Centella asiatica.\n","PeriodicalId":10850,"journal":{"name":"Current Biotechnology","volume":"29 1","pages":"120-126"},"PeriodicalIF":0.0000,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phytochemical Analysis of Secondary Metabolites and Evaluation of the Antioxidant Activity of Centella asiatica (L.) by UV-Vis Spectroscopy\",\"authors\":\"Shouvik Chakravarty, K. Sultana, Sumanta Das, Indrani Chandra\",\"doi\":\"10.2174/2211550109999200821155854\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n\\nCentella asiatica (L.) is an herbaceous, frost-tender perennial plant belonging\\nto the Apiaceae family. It is rich in important secondary metabolites such as terpenoids, flavonoids and\\nsaponins. It is used as a traditional medicine for skin infection, cough and asthma.\\n\\n\\n\\nThe present study was intended at the quantitative and qualitative analysis of its secondary\\nmetabolites. Thin Layer Chromatography (TLC) was performed to separate the compounds according\\nto their Rf (Retention factor) values.\\n\\n\\n\\nThe total phenolic content of ethanol leaf extract was 45.72 ± 6.05 mg GAE/g determined by\\nFolin Ciocalteu’s assay. The maximum content of total flavonoids was found in aqueous leaf extract,\\n44.62 ± 2.45 mg QE/g. Aqueous extract of leaf and stem showed the highest antioxidant activity with\\nDPPH (2,2- diphenyl-1-picrylhydrazyl) scavenging assay (45.66 ± 1.29 & 45.20 ± 3.0, respectively).\\n\\n\\n\\nThe present study revealed the antioxidant properties in Centella asiatica.\\n\",\"PeriodicalId\":10850,\"journal\":{\"name\":\"Current Biotechnology\",\"volume\":\"29 1\",\"pages\":\"120-126\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-11-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Biotechnology\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://doi.org/10.2174/2211550109999200821155854\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Biotechnology","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.2174/2211550109999200821155854","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Phytochemical Analysis of Secondary Metabolites and Evaluation of the Antioxidant Activity of Centella asiatica (L.) by UV-Vis Spectroscopy
Centella asiatica (L.) is an herbaceous, frost-tender perennial plant belonging
to the Apiaceae family. It is rich in important secondary metabolites such as terpenoids, flavonoids and
saponins. It is used as a traditional medicine for skin infection, cough and asthma.
The present study was intended at the quantitative and qualitative analysis of its secondary
metabolites. Thin Layer Chromatography (TLC) was performed to separate the compounds according
to their Rf (Retention factor) values.
The total phenolic content of ethanol leaf extract was 45.72 ± 6.05 mg GAE/g determined by
Folin Ciocalteu’s assay. The maximum content of total flavonoids was found in aqueous leaf extract,
44.62 ± 2.45 mg QE/g. Aqueous extract of leaf and stem showed the highest antioxidant activity with
DPPH (2,2- diphenyl-1-picrylhydrazyl) scavenging assay (45.66 ± 1.29 & 45.20 ± 3.0, respectively).
The present study revealed the antioxidant properties in Centella asiatica.
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