{"title":"苏云金芽孢杆菌Kfr-24菌株的特性及对小夜蛾的毒力研究","authors":"H. A. Ahmed, Salah G. Ali, U. Abdul-Raouf","doi":"10.0001/(aj).v3i0.723","DOIUrl":null,"url":null,"abstract":"Under these studies Bacillus thuringiensis wettable powder comparable to standard strain, there are only one isolate which confirmed by molecular characterization. Scanning electron microscopy observed that the presence of cuboidal crystals in Bacillus thuringiensis Alex-13, and observed bipyramidal crystals in case of Bacillus thuringiensis HD-1, as standard strain. Polymerase chain reactions (PCR) revealed the presence of Cry1-like sequences in addition to standard strain, which confirmed by gel electrophoresis. Also, during these studies.Bioassay recorded that the highest mortality rate by determination of LC50 for 5 days against Spodoptera littolaris third instars larvae were 69, 86, and 100(%) in case of Bt Alex-13, Bt-HD-1 standard strain and Bt commercial wettable powder, respectively.","PeriodicalId":7790,"journal":{"name":"Anglisticum Journal","volume":"7 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2016-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization and Toxicity of Bacillus Thuringiensis Strain Kfr-24, And Commercial Formulations against Spodoptera Littolaris\",\"authors\":\"H. A. Ahmed, Salah G. Ali, U. Abdul-Raouf\",\"doi\":\"10.0001/(aj).v3i0.723\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Under these studies Bacillus thuringiensis wettable powder comparable to standard strain, there are only one isolate which confirmed by molecular characterization. Scanning electron microscopy observed that the presence of cuboidal crystals in Bacillus thuringiensis Alex-13, and observed bipyramidal crystals in case of Bacillus thuringiensis HD-1, as standard strain. Polymerase chain reactions (PCR) revealed the presence of Cry1-like sequences in addition to standard strain, which confirmed by gel electrophoresis. Also, during these studies.Bioassay recorded that the highest mortality rate by determination of LC50 for 5 days against Spodoptera littolaris third instars larvae were 69, 86, and 100(%) in case of Bt Alex-13, Bt-HD-1 standard strain and Bt commercial wettable powder, respectively.\",\"PeriodicalId\":7790,\"journal\":{\"name\":\"Anglisticum Journal\",\"volume\":\"7 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-01-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anglisticum Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.0001/(aj).v3i0.723\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anglisticum Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.0001/(aj).v3i0.723","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
在这些研究中，苏云金芽孢杆菌可湿性粉剂与标准菌株相当，只有一株经分子鉴定得到证实。扫描电镜观察到苏云金芽孢杆菌alex1 -13中存在立方晶体，苏云金芽孢杆菌HD-1中存在双锥体晶体。聚合酶链反应(PCR)显示，除标准菌株外，还存在cry1样序列，凝胶电泳证实了这一点。同时，在这些研究中。生物测定结果显示，Bt alex13、Bt hd -1标准品系和Bt可湿性商业粉剂对小夜蛾3龄幼虫5 d的LC50死亡率最高，分别为69%、86%和100%。
Characterization and Toxicity of Bacillus Thuringiensis Strain Kfr-24, And Commercial Formulations against Spodoptera Littolaris
Under these studies Bacillus thuringiensis wettable powder comparable to standard strain, there are only one isolate which confirmed by molecular characterization. Scanning electron microscopy observed that the presence of cuboidal crystals in Bacillus thuringiensis Alex-13, and observed bipyramidal crystals in case of Bacillus thuringiensis HD-1, as standard strain. Polymerase chain reactions (PCR) revealed the presence of Cry1-like sequences in addition to standard strain, which confirmed by gel electrophoresis. Also, during these studies.Bioassay recorded that the highest mortality rate by determination of LC50 for 5 days against Spodoptera littolaris third instars larvae were 69, 86, and 100(%) in case of Bt Alex-13, Bt-HD-1 standard strain and Bt commercial wettable powder, respectively.
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