榕树抗氧化活性测定及酚类黄酮含量测定。F)水果

Yamin Yamin, Andriani Rina, Sabarudin Sabarudin, Haijah Nur, Kasmawati Henny
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摘要

背景:印尼传统药物中使用的植物之一。F)传统上用于治疗一些疾病,包括疟疾、腹泻、糖尿病、止痛、抗真菌、痢疾、驱虫药、抗氧化剂、止血和抗炎。目的:研究荔枝果实潜在的体外抗氧化活性测定及植物化学成分含量。方法:采用浸渍法提取枸杞叶粉,用ABTS阳离子和CUPRAC自由基评价其抗氧化活性。用Folin-Ciocalteau法测定总酚含量。同时,采用氯化铝络合比色法测定总黄酮含量;结果:竹叶榕提取液及提取物。水果具有最强的抗氧化潜力。乙酸乙酯部位对ABTS和CUPRAC自由基的抗氧化活性最强,IC50值分别为6.33±0.01µg/mL和11.64±0.28µg/mL。乙酸乙酯部位的酚类和类黄酮含量也较高,分别为28±0.05 mg GAE/100 mg样品和43.08±0.48 mg QE/100 mg样品。结论:乙酸乙酯部分具有作为天然抗氧化剂的潜力和作为营养保健品的潜力。
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Antioxidant activity assay and determination of phenolic and flavonoid content of Libho (Ficus Septica Burm. F) fruits
Background: One of the plants used in Indonesian traditional medicine Libho (Ficus Septica Burm. F) is traditionally used to treat some diseases, including malaria, diarrhea, diabetes, analgesic, antifungal, dysentery, anthelmintic, antioxidant, hemostatic and anti-inflammatory. Purpose: The purpose of the study is to investigate the potential in vitro antioxidant activity assay and phytochemical content of Libho fruits. Methods: Libho leaves powder was extracted with the maceration method Antioxidant activity was evaluated using ABTS cation and CUPRAC radicals. Total phenolic content was determined using the Folin-Ciocalteau method. Meanwhile, the total flavonoid content was determined using the aluminum chloride complex colorimetric method; Results: Extract and fraction of Ficus Septica Burm. F fruits have the strongest antioxidant potential. The ethyl acetate fraction showed the strongest antioxidant activity on both ABTS and CUPRAC radicals with IC50 values of 6.33 ± 0.01 µg/mL and 11.64 ± 0.28 µg/mL, respectively. Ethyl acetate fraction also showed high phenolic and flavonoid content with values of 28 ± 0.05 mg GAE/100 mg sample and 43.08 ± 0.48 mg QE/100 mg sample, respectively. Conclusion: Ethyl acetate fraction has the potential to be used as a source of natural antioxidants and has the potential to be used as a nutraceutical.
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