{"title":"用数码光学显微镜研究活细胞的分子生物学","authors":"Paolo Gualtieri","doi":"10.1016/0739-6260(92)90028-C","DOIUrl":null,"url":null,"abstract":"<div><p>The intention of this review is to introduce to microscopists some of the fundamental limits as well as the advances made in digital optical microscopy techniques, also to show their potentialities in the field of molecular cell biology. Several items within the wide field of this subject will be discussed, such as geometric and photometric features, photophysics, statistics of photon flux and detection, spatial and intensity characteristics of digital images, their manipulation and display, fluorescent probes, and measuring techniques.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 3","pages":"Pages 239-257"},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90028-C","citationCount":"16","resultStr":"{\"title\":\"Molecular biology in living cells by means of digital optical microscopy\",\"authors\":\"Paolo Gualtieri\",\"doi\":\"10.1016/0739-6260(92)90028-C\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The intention of this review is to introduce to microscopists some of the fundamental limits as well as the advances made in digital optical microscopy techniques, also to show their potentialities in the field of molecular cell biology. Several items within the wide field of this subject will be discussed, such as geometric and photometric features, photophysics, statistics of photon flux and detection, spatial and intensity characteristics of digital images, their manipulation and display, fluorescent probes, and measuring techniques.</p></div>\",\"PeriodicalId\":100925,\"journal\":{\"name\":\"Micron and Microscopica Acta\",\"volume\":\"23 3\",\"pages\":\"Pages 239-257\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0739-6260(92)90028-C\",\"citationCount\":\"16\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Micron and Microscopica Acta\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/073962609290028C\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Micron and Microscopica Acta","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/073962609290028C","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular biology in living cells by means of digital optical microscopy
The intention of this review is to introduce to microscopists some of the fundamental limits as well as the advances made in digital optical microscopy techniques, also to show their potentialities in the field of molecular cell biology. Several items within the wide field of this subject will be discussed, such as geometric and photometric features, photophysics, statistics of photon flux and detection, spatial and intensity characteristics of digital images, their manipulation and display, fluorescent probes, and measuring techniques.
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