Lamplaimas Tangpan, Tippawadee Jansai, Sasiwan Kerdpoo, Tiemjan Kiewkarnkha, Ekthong Limweeraprajak, T. Tatu
{"title":"抗γ-珠蛋白链单克隆抗体;Thal N/B，准确测定SEA-α地中海贫血1型性状中的F细胞","authors":"Lamplaimas Tangpan, Tippawadee Jansai, Sasiwan Kerdpoo, Tiemjan Kiewkarnkha, Ekthong Limweeraprajak, T. Tatu","doi":"10.1080/15321819.2019.1671862","DOIUrl":null,"url":null,"abstract":"ABSTRACT The monoclonal antibody (mAb) against γ-globin chain clone Thal N/B was produced. It was aimed at measuring the quantity of HbF (α2γ2)-containing red blood cells or F cells by flow cytometry. However, it may cross-react with Hb Bart’s (γ4) which is present in the SEA-α thalassemia 1 trait. We measured FC levels by flow cytometry using this in-house mAb for 100 blood samples. Prevalence of high FC trait in this cohort was 51%. Ten of 12 SEA-α thalassemia 1 trait were included in the 51% high FC individuals. Comparing FC levels in the 51 high FC individuals having and not having the SEA-α thalassemia 1 trait showed no difference of FC levels. It was concluded that Hb Bart’s did not interfere with FC measurement by flow cytometry using the in-house Thal N/B mAb. Therefore, it can be used for measuring F cell levels in regions having a high prevalence of the SEA-α thalassemia 1 trait. The findings of this research should apply to other clones of anti-γ-globin chain mAb that are aimed for HbF/FC quantification.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The in-house monoclonal antibody against γ-globin chain; Thal N/B, accurately measured F cells in SEA-α thalassemia 1 trait\",\"authors\":\"Lamplaimas Tangpan, Tippawadee Jansai, Sasiwan Kerdpoo, Tiemjan Kiewkarnkha, Ekthong Limweeraprajak, T. Tatu\",\"doi\":\"10.1080/15321819.2019.1671862\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT The monoclonal antibody (mAb) against γ-globin chain clone Thal N/B was produced. It was aimed at measuring the quantity of HbF (α2γ2)-containing red blood cells or F cells by flow cytometry. However, it may cross-react with Hb Bart’s (γ4) which is present in the SEA-α thalassemia 1 trait. We measured FC levels by flow cytometry using this in-house mAb for 100 blood samples. Prevalence of high FC trait in this cohort was 51%. Ten of 12 SEA-α thalassemia 1 trait were included in the 51% high FC individuals. Comparing FC levels in the 51 high FC individuals having and not having the SEA-α thalassemia 1 trait showed no difference of FC levels. It was concluded that Hb Bart’s did not interfere with FC measurement by flow cytometry using the in-house Thal N/B mAb. Therefore, it can be used for measuring F cell levels in regions having a high prevalence of the SEA-α thalassemia 1 trait. The findings of this research should apply to other clones of anti-γ-globin chain mAb that are aimed for HbF/FC quantification.\",\"PeriodicalId\":15987,\"journal\":{\"name\":\"Journal of Immunoassay and Immunochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Immunoassay and Immunochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/15321819.2019.1671862\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Immunoassay and Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/15321819.2019.1671862","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The in-house monoclonal antibody against γ-globin chain; Thal N/B, accurately measured F cells in SEA-α thalassemia 1 trait
ABSTRACT The monoclonal antibody (mAb) against γ-globin chain clone Thal N/B was produced. It was aimed at measuring the quantity of HbF (α2γ2)-containing red blood cells or F cells by flow cytometry. However, it may cross-react with Hb Bart’s (γ4) which is present in the SEA-α thalassemia 1 trait. We measured FC levels by flow cytometry using this in-house mAb for 100 blood samples. Prevalence of high FC trait in this cohort was 51%. Ten of 12 SEA-α thalassemia 1 trait were included in the 51% high FC individuals. Comparing FC levels in the 51 high FC individuals having and not having the SEA-α thalassemia 1 trait showed no difference of FC levels. It was concluded that Hb Bart’s did not interfere with FC measurement by flow cytometry using the in-house Thal N/B mAb. Therefore, it can be used for measuring F cell levels in regions having a high prevalence of the SEA-α thalassemia 1 trait. The findings of this research should apply to other clones of anti-γ-globin chain mAb that are aimed for HbF/FC quantification.
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