Effects of synthesized silver and chitosan nanoparticles using Nerium oleander and Aloe vera on antioxidant enzymes in Musca domestica

Green synthesis of silver and chitosan nanoparticles have been achieved using Nerium.oleander and Aloe vera leaf extract as reducing agents. The formation of nanoparticles was quantified by UV-vis spectroscopy of wave length from 200-700. TEM, XRD and FTIR were used for characterization. The present investigation was carried out to assess the activity of antioxidant enzymes, SOD, CAT, GPx and GST in third instar larvae of Musca domestica under the effect of methanol leaf extract, synthesized silver and chitosan nanoparticles and the LC50 was used for biochemical assay. The activity of antioxidant enzymes in M. domestica larvae were varied with the time where an increase in SOD activity was recorded after 24hrs in methanol extract and AgNPs treated larvae, then decreased again after 48hrs. In CsNPs treated larvae, SOD induced a continuously decreased activity at all times of investigation. CAT activity followed the same trend of SOD. After 48hrs of treatment the activity of GPs was significantly decreased in all treated groups, at 1% and 5% level of significance in larvae treated with chitosan Nerium and chitosan A. vera, respectively.  After an increase in GST activity at 24hrs, a decrease was noticed after 48hrs. From the present study, the use of N. oleander and A. vera as methanol crude extracts to synthesize silver and chitosan nanoparticles could be a new approach for the control of house flies.