一种小叶蝉(Lagenaria siceraria var. microcarpa)叶绿体全基因组的研究Hara

Zhaohui Zhang, Dandan Xi, Jie Zhao, Xiaofeng Li, Lu Gao, Chunhong Chen, Yuying Zhu, Hongfang Zhu
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引用次数: 0

摘要

小葫芦(Lagenaria siceraria var。microcarpa (Naud)。原草属Lagenaria科的一个栽培品种,是重要的药用和观赏植物。然而,对其叶绿体基因组的研究却很少。在本研究中,我们用高通量测序方法对其叶绿体(cp)全基因组进行了鉴定。圆形cp基因组的长度为157,121 bp,由一对反向重复区分隔出一个长单拷贝和一个小单拷贝。共标注了132个基因,其中蛋白编码基因86个,tRNA基因37个,rRNA基因8个,伪基因1个。cp基因组总GC含量为37.09%。最大似然树分析表明,小葫芦与丝瓜有亲缘关系。这些结果为进一步了解小葫芦cp基因组提供了依据,为今后的遗传研究提供了参考。
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The Complete Chloroplast Genome of a Variety of Lagenaria siceraria var. microcarpa (Naud.) Hara
Small gourd ( Lagenaria siceraria  var .  microcarpa  (Naud.) Hara), a cultivar of  Lagenaria  family, is an important medicinal and ornamental plants. However, there are few studies on its chloroplast (cp) genome. In this study, we characterized its complete chloroplast (cp) genome with high throughput sequencing. The circular cp genome is determined to be 157, 121 bp in length, with a long sing-copy and a small sing-copy separated by a pair of inverted repeat regions. A total of 132 genes were annotated, including 86 protein-coding genes, 37 tRNA genes, 8 rRNA genes, and 1 pseudo gene. The overall GC content of cp genome was 37.09%. The maximum likelihood tree suggested that small gourd was closely related to  Lagenaria siceraria.  These results provided an understanding of small gourd cp genome and might be useful for future genetic studies.
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