筛选麦加省不同沙漠土壤检测新型抗生素产放线菌

Abdullah AI Hausawi, N. Baeshen, A. Al-Hejin, Mohammed A. AlMatary, Saleh M. Almaaqar, Mohamed Morsi Mohamed Ahmed
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摘要

本研究的目的是利用生物化学、形态学和16S rRNA基因测序技术,从散布在麦加地区的一些植物周围的沙漠土壤中分离、纯化和表征放线菌,并对对某些病原体有活性的物种进行分类研究,并研究提取的抗生素的特征和性质。土壤样本是从沙特阿拉伯麦加地区的三个地点收集的。它们被带到实验室,用放线菌酸盐分离培养基在27°C下培养4天。基于生理、形态和16S rRNA基因序列,我们获得了8个样本:螺旋链霉菌、棘状链霉菌、毛状链霉菌、卡平链霉菌、吉斯链霉菌、jakartensis链霉菌、levis链霉菌和atacamensis链霉菌。所有分离株链霉菌均对肺炎链球菌、耐甲氧西林金黄色葡萄球菌、金黄色葡萄球菌、化脓性链球菌具有抗菌作用,并对黑曲霉和白色念珠菌具有抗菌作用。这些分离株具有抗菌效果,可用于制造药用或农业应用的新型抗生素。
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Screening Different Desert Soils in Makkah Province to Detect Novel Antibiotics Producing Actinomycetes
The purpose of this study was to isolate, purify, and characterize actinomycetes from desert soil surrounding some plants scattered in Makkah Region using biochemical, morphological, and 16S rRNA gene sequencing techniques, as well as to conduct a taxonomic study of species that show activity against some pathogens and to study the characteristics and properties of antibiotics extracted. Soil samples were collected from three locations in Saudi Arabia's Makkah region. They were brought to the lab and cultured for four days at 27° C using actinomistate isolation media. We obtained eight samples based on physiological, morphological, and 16S rRNA gene sequences: Streptomyces spiralis, Streptomyces spinoverrucosus, Streptomyces fimbriatus, Streptomyces carpinensis, Streptomyces geysiriensis, Streptomyces djakartensis, Streptomyces levis, and Streptomyces atacamensis. All isolates of Streptomyces demonstrated antibacterial efficacy against Streptococcus pneumonia, Methicillin-resistant Staphylococcus aureus, Staphylococcus aureus, Streptococcus pyogenes, and antifungal including Aspergillus niger and Candida albicans. These isolates had antimicrobial efficacy and might be used to create novel antibiotics for medicinal or agricultural application.
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