Rohman Mohammad Saifur, Sishartami Lintang Widya, Widodo Nashi, Rachmawati Ermin, Lukitasari Mifetika
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The Combination of Decaffeinated Coffee (Coffea canephora) and Green Tea (Camellia sinensis) Reduced PSGL-1 Glycosylation by GALNT2 in Ox-LDL-induced RAW 264.7
Context: Coffee and green tea extract combination is expected to reduce macrophage migration. Aims: We investigated the effects of combination of coffee and green tea extracts on GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells. Materials and methods: RAW 264.7 cells were treated with a combination of coffee and green tea extracts with three different doses for 24 hours (coffee 80 μg/mL + green tea 80 μg/mL; coffee 160 μg/mL + green tea 160 μg/mL; coffee 320 μg/mL + green tea 320 μg/mL), respectively. Then, cells stimulated with 8 μg/mL Ox-LDL for 20 hours. GALNT2 mRNA expression was analyzed by reverse transcription polymerase chain reaction and western blot for PSGL-1 protein expression. Results: GALNT2 gene expression and PSGL-1 protein expression decreased significantly (P < 0.05) with treatment by combination of coffee and green tea extracts in dose-dependent manner. Conclusion: Coffee and green tea extract combination administration significantly reduced GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells.
期刊介绍：
The International Journal of Nutrition, Pharmacology, Neurological Diseases (IJNPND) is an international, open access, peer reviewed journal which covers all fields related to nutrition, pharmacology, neurological diseases. IJNPND was started by Dr. Mohamed Essa based on his personal interest in Science in 2009. This journal doesn’t link with any society or any association. The co-editor-in chiefs of IJNPND (Prof. Gilles J. Guillemin, Dr. Abdur Rahman and Prof. Ross grant) and editorial board members are well known figures in the fields of Nutrition, pharmacology, and neuroscience. First, the journal was started as two issues per year, then it was changed into 3 issues per year and since 2013, it publishes 4 issues per year till now. This shows the slow and steady growth of this journal. To support the reviewers and editorial board members, IJNPND offers awards to the people who does more reviews within one year. The International Journal of Nutrition, Pharmacology, Neurological Diseases (IJNPND) is published Quarterly. IJNPND has three main sections, such as nutrition, pharmacology, and neurological diseases. IJNPND publishes Research Papers, Review Articles, Commentaries, case reports, brief communications and Correspondence in all three sections. Reviews and Commentaries are normally commissioned by the journal, but consideration will be given to unsolicited contributions. International Journal of Nutrition, Pharmacology, Neurological Diseases is included in the UGC-India Approved list of journals.
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