{"title":"小鼠脑中金属硫蛋白iii的选择性和简单定量","authors":"A. Yasutake, M. Satoh, C. Tohyama, K. Hirayama","doi":"10.1248/JHS.45.222","DOIUrl":null,"url":null,"abstract":"For the selective quantification of metallothionein (MT)-III, MT samples from mouse brain were converted to Hg-MT, then chromatographed using a double (connected-in-tandem) FPLC-gel permeation column system. The chromatogram showed two peaks with a slight overlap. Hg-MT-III was eluted prior to Hg-MT-I and II, probably due to a slightly higher molecular weight. The amount of each isomer was determined from an analysis of the Hg content in each fraction. The present procedure revealed that MT-III in the mouse brain was comparable in quantity to MT-I and II. Thus, MT-III in mouse brain could be selectively quantified at a sub-picomol level.","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"55 1","pages":"222-225"},"PeriodicalIF":0.0000,"publicationDate":"1999-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":"{\"title\":\"SELECTIVE AND SIMPLE QUANTIFICATION OF METALLOTHIONEIN III IN MOUSE BRAIN\",\"authors\":\"A. Yasutake, M. Satoh, C. Tohyama, K. Hirayama\",\"doi\":\"10.1248/JHS.45.222\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"For the selective quantification of metallothionein (MT)-III, MT samples from mouse brain were converted to Hg-MT, then chromatographed using a double (connected-in-tandem) FPLC-gel permeation column system. The chromatogram showed two peaks with a slight overlap. Hg-MT-III was eluted prior to Hg-MT-I and II, probably due to a slightly higher molecular weight. The amount of each isomer was determined from an analysis of the Hg content in each fraction. The present procedure revealed that MT-III in the mouse brain was comparable in quantity to MT-I and II. Thus, MT-III in mouse brain could be selectively quantified at a sub-picomol level.\",\"PeriodicalId\":14851,\"journal\":{\"name\":\"Japanese journal of toxicology and environmental health\",\"volume\":\"55 1\",\"pages\":\"222-225\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-08-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese journal of toxicology and environmental health\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1248/JHS.45.222\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese journal of toxicology and environmental health","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1248/JHS.45.222","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
SELECTIVE AND SIMPLE QUANTIFICATION OF METALLOTHIONEIN III IN MOUSE BRAIN
For the selective quantification of metallothionein (MT)-III, MT samples from mouse brain were converted to Hg-MT, then chromatographed using a double (connected-in-tandem) FPLC-gel permeation column system. The chromatogram showed two peaks with a slight overlap. Hg-MT-III was eluted prior to Hg-MT-I and II, probably due to a slightly higher molecular weight. The amount of each isomer was determined from an analysis of the Hg content in each fraction. The present procedure revealed that MT-III in the mouse brain was comparable in quantity to MT-I and II. Thus, MT-III in mouse brain could be selectively quantified at a sub-picomol level.
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