马鞭草科白皮体外抗氧化及自由基清除活性的研究

Md Omar Ali, N. A. Khatune, M. S. Parvin, A. Alam, Md Aziz Abdur Rahman
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引用次数: 1

摘要

研究了马鞭草科植物Lippia alba (Mill.)的水乙醇提取物和各组分的多酚含量和抗氧化活性。用5%的水乙醇提取干燥的course粉末,得到乙醇提取物(LAE),依次用正己烷(LAH)、氯仿(LAC)、乙酸乙酯(LAA)和水(LAQ)进行分割。测定各提取物的多酚含量和抗氧化活性。LAE、LAH、LAC、LAA和LAQ的酚类含量分别为97.463±0.36、55.030±0.690、91.343±0.120、117.340±1.349、81.630±0.550、418.470±4.962、583.847±2.274、168.623±1.186、161.970±1.793、392.257±4.856 mg /g。结果表明,与其他馏分相比,LAA中酚类物质含量最高,LAH中总黄酮含量最高。LAE、LAH、LAC、LAA、LAQ和标准品(100 μg/ml)的黄酮醇含量和原花青素含量分别为0.611±0.01、0.144±0.031、1.938±0.023、1.944±0.049、0.385±0.007和0.984±0.025和0.087±0.005、0.155±0.004、0.068±0.002、0.049±0.002、0.056±0.003和1.533±0.044,表明LAC和LAA的黄酮含量显著高于标准品。然而,提取物中的原花青素含量低于标准儿茶素。LAE、LAH、LAC、LAA、LAQ、standard的吸光度分别为0.667±0.005、0.753±0.046、0.444±0.009、0.409±0.005、0.235±0.007、1.534±0.38、1.633±0.055、0.258±0.033、3.839±0.051、2.301±0.004、0.360±0.042、1.046±0.053。结果表明,各提取液均具有较好的抗氧化活性。在DPPH自由基清除试验和羟基自由基清除试验中,LAE、LAH、LAC、LAA、LAQ和standard的IC50分别为15.73±0.51、58.90±1.86、15.93±1.24、3.84±0.18、7.17±0.36和3.48±0.17 μg/ml和10.95±0.560、13.25±0.56、11.69±0.69、5.68±0.408、6.36±0.581和6.50±0.271 μg/ml。在LAA中发现了与标准BHT和儿茶素相似的显著清除活性。所有这些结果表明,植物白桦,特别是氯仿和乙酸乙酯部分可能是抗氧化铅的良好来源。孟加拉国医药杂志26(1):7- 14,2023(1月)
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In vitro Antioxidant and Free Radical Scavenging Activity of Lippia alba (Verbenaceae)
The research was designated to investigate the polyphenolic contents and antioxidant activity of the aqueous ethanolic extract and various fractions from the plant Lippia alba (Mill.) (Family: Verbenaceae). The dried course powder was extracted with 5% aqueous ethanol to get ethanolic extract (LAE) that was partitioned successively with n-hexane (LAH), chloroform (LAC), ethyl acetate (LAA), and water (LAQ). All the extracts were tested for polyphenol contents and antioxidant activity. The phenolic contents and the flavonoid contents of LAE, LAH, LAC, LAA, and LAQ were found to be 97.463±0.36, 55.030±0.690, 91.343±0.120, 117.340±1.349 and 81.630±0.550 and 418.470±4.962, 583.847±2.274, 168.623±1.186, 161.970±1.793 and 392.257±4.856 mg of GAE/g of dried extractives, respectively. The data showed that LAA contained highest phenolics and LAH contains highest flavonoids content than that of other fractions. The flavonol contents and the proanthocyanidin contents of LAE, LAH, LAC, LAA, LAQ and standard (at 100 μg/ml each) were 0.611±0.01, 0.144±0.031, 1.938±0.023, 1.944±0.049, 0.385±0.007 and 0.984±0.025 and 0.087±0.005, 0.155±0.004, 0.068±0.002, 0.049±0.002, 0.056±0.003 and 1.533±0.044, respectively, indicating remarkable flavonoid contents of LAC and LAA when compare to standard. However, proanthocyanidin contents of the extractives are less than standard catechin. In total antioxidant activity and reducing power capacity assay LAE, LAH, LAC, LAA, LAQ and standard showed absorbance at 0.667±0.005, 0.753±0.046, 0.444±0.009, 0.409±0.005, 0.235±0.007 and 1.534±0.38 and 1.633±0.055, 0.258±0.033, 3.839±0.051, 2.301±0.004, 0.360±0.042, and 1.046±0.053, respectively. The results demonstrated that all the extractives of L. alba had appreciable antioxidant activity when compared to standard. In DPPH radical scavenging assay and hydroxyl radical scavenging assay, the IC50 of LAE, LAH, LAC, LAA, LAQ and standard were found to be 15.73±0.51, 58.90±1.86, 15.93±1.24, 3.84±0.18, 7.17±0.36 and 3.48±0.17 μg/ml and 10.95±0.560, 13.25±0.56, 11.69±0.69, 5.68±0.408, 6.36±0.581 and 6.50±0.271 μg/ml, respectively. The significant scavenging activity was found in LAA that was similar to standard BHT and catechin. All these observations demonstrated that the plant L. alba specially chloroform and ethyl acetate fractions might be a good source for antioxidative lead. Bangladesh Pharmaceutical Journal 26(1): 7-14, 2023 (January)
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