T. Murata, Satoshi Amarume, T. Usui, H. Ohshima, K. Abukawa, Bunji Kanzaki, A. Hosono
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Measurement of lactose in bovine raw milk using oligosaccharide oxidase
A novel method for measuring lactose in bovine raw milk has been developed. The method is based on measuring H2O2 produced from lactose by oligosaccharide oxidase through a coupled reaction involving peroxidase with a chromogenic reagent. The assay system gave a linear dose response of Lactose in the range of 25-300 μg/mL in 2-20 min of incubation. Lactose concentration in diluted bovine raw milk was directly determined with high precision by this method without any pretreatment. This is a quick and simple procedure and is suitable for multi-sample assay compared to the conventional Lane-Eynon method.
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