Modification in Extra Cellular Polymeric Substances of Staphylococcus aureus Using Sesbania grandiflora

Microorganism secret high molecular weight compound called as extra cellular polymeric substance (EPS) which is attached on the outer surface of the bacteria which is mostly release during its log phase. Bacterial EPS have been identified as one of the major components in biofilms [1]. Biofilms are formed with interaction among microbial aggregates, filamentous bacterial strains, organic and inorganic particles, which are held together by EPS [2]. The biofilms are complex structure where microbial cell aggregates are embedded inside a hydrated matrix made up of extra cellular polymeric substances. EPS majorly comprised of four substance called carbohydrate, protein, lipids and environmental DNA (e DNA). This biofilm acts as a protective barrier and provides resistance against antibiotics, degrading enzymes, protozoan grazers and host immune response [3].