FIA-Spectrophotometric Determination of Ascorbic Acid in Rat's Lung by Reduction of Iron(III)

Abstract A FIA indirect spectrophotometric determination of ascorbic acid (vitamin C) was developed using its reducing action on Fe(III) and following the spectrophotometric determination of the reduced ion by 2,2′-dipyridyl and monitoring the absorbance signal at 520 nm. The procedure is based on the reduction of iron(III) to iron(II) with ascorbic acid. The iron(II) obtained is then able to react with 2,2′-dipyridyl in order to form a colored complex. The linear range of the method was 1.0–10.0 ppm ascorbic acid. The proposed method was sensitive, rapid (40 samples/h) and reproducible (relative standard deviation 1.2%, n = 40). Satisfactory results were obtained in the determination of ascorbic acid in rat's lung.