Comparative Analysis of Tyrosine tRNA (MT-TY) Gene Mutation Related to Lung Cancer Patients from District Peshawar, Khyber-Pakhtunkhwa, Pakistan During 2020-2022

The uncontrolled growth of cells in the lungs is called lung cancer (LC). Majority of recorded LC cases is as a result of cigarette smoking. It is one of the most occurring types of cancer around the globe. Objective: To emphasizes on amplification of specific mitochondrial DNA (mtDNA) genes coding for tyrosine tRNA and to find out its association with LC patients of Peshawar. Methods: In 18 LC patients, the mitochondrially encoded tRNA tyrosine gene was studied. Samples were collected from the Institute of Radiotherapy & Nuclear Medicine (IRNUM), Peshawar, Khyber Pakhtunkhwa. The participants varied in age from 12 to 86 years. Patients had diabetes, hypertension, hepatitis C, bronchial asthma, dyspnea, hemoptysis, cardio myopathy, and stomach ulcers. DNA extraction and PCR of the tyr tRNA gene were performed. Along with gel electrophoresis. 8 samples were cleaned and sequenced. Results: The results were used for comparison with the reference DNA sequence of mitochondria i.e., revised Cambridge Reference Sequence (rCRS) Accession No 0129201. According to NCBI BLAST data, there were no variations in the nucleotide position 5826-5891 of the mitochondrially encoded tyrosine tRNA gene in LC subject. Chromatogram of tyrosine gene which was taken from UGENE software showed that there is no variation in Tyrosine gene subject sequence. Conclusions: We found no mutation in the mtDNA encoded tyr tRNA gene related to LC patient from District Peshawar Khyber-Pakhtunkhwa, Pakistan during 2020-2022.