{"title":"荧光和表面增强拉曼散射双模式PCR检测异角细辛DNA条形码","authors":"Chunhao WEI, Aihong WU, Lingling GUO, Liqiang LIU, Chuanlai XU, Hua KUANG, Xinxin XU","doi":"10.1016/j.cjac.2023.100304","DOIUrl":null,"url":null,"abstract":"<div><p>Asarum plants containing many functional substances are widely used in traditional prescriptions and tonics. DNA barcoding is a relatively short specific gene segment in the genome which can be used for species identification purposes. The present work selected RNA transcriber type II intron shear enzyme gene (matK) as the target and developed a novel fluorescence and surface enhanced Raman scattering (SERS) dual-model PCR for the detection of <em>Asarum heterotropoides</em> (AH) in plants. The dual-model method achieved specific AH DNA detection with a limit of detection (LOD) of 10<sup>−3</sup> ng/µL for the fluorescence signal and 10<sup>−4</sup> ng/µL for the SERS signal in the range of 10<sup>−4</sup>−10 ng/µL. Agarose gel electrophoresis results and qPCR results showed the novel method was highly specific for AH detection and only the AH was successfully amplified.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100304"},"PeriodicalIF":1.2000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000786/pdfft?md5=f75c2129821405a2c0004102d1aa101f&pid=1-s2.0-S1872204023000786-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Fluorescence and surface enhanced Raman scattering dual-model PCR for the detection of DNA barcoding of Asarum heterotropoides\",\"authors\":\"Chunhao WEI, Aihong WU, Lingling GUO, Liqiang LIU, Chuanlai XU, Hua KUANG, Xinxin XU\",\"doi\":\"10.1016/j.cjac.2023.100304\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Asarum plants containing many functional substances are widely used in traditional prescriptions and tonics. DNA barcoding is a relatively short specific gene segment in the genome which can be used for species identification purposes. The present work selected RNA transcriber type II intron shear enzyme gene (matK) as the target and developed a novel fluorescence and surface enhanced Raman scattering (SERS) dual-model PCR for the detection of <em>Asarum heterotropoides</em> (AH) in plants. The dual-model method achieved specific AH DNA detection with a limit of detection (LOD) of 10<sup>−3</sup> ng/µL for the fluorescence signal and 10<sup>−4</sup> ng/µL for the SERS signal in the range of 10<sup>−4</sup>−10 ng/µL. Agarose gel electrophoresis results and qPCR results showed the novel method was highly specific for AH detection and only the AH was successfully amplified.</p></div>\",\"PeriodicalId\":277,\"journal\":{\"name\":\"Chinese Journal of Analytical Chemistry\",\"volume\":\"51 9\",\"pages\":\"Article 100304\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1872204023000786/pdfft?md5=f75c2129821405a2c0004102d1aa101f&pid=1-s2.0-S1872204023000786-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Journal of Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1872204023000786\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1872204023000786","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Fluorescence and surface enhanced Raman scattering dual-model PCR for the detection of DNA barcoding of Asarum heterotropoides
Asarum plants containing many functional substances are widely used in traditional prescriptions and tonics. DNA barcoding is a relatively short specific gene segment in the genome which can be used for species identification purposes. The present work selected RNA transcriber type II intron shear enzyme gene (matK) as the target and developed a novel fluorescence and surface enhanced Raman scattering (SERS) dual-model PCR for the detection of Asarum heterotropoides (AH) in plants. The dual-model method achieved specific AH DNA detection with a limit of detection (LOD) of 10−3 ng/µL for the fluorescence signal and 10−4 ng/µL for the SERS signal in the range of 10−4−10 ng/µL. Agarose gel electrophoresis results and qPCR results showed the novel method was highly specific for AH detection and only the AH was successfully amplified.
期刊介绍：
Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.
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